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Biological Applications of Chromophores With Large Two-Photon Cross Sections

Published online by Cambridge University Press:  02 July 2020

M.E. Dickinson ,
D. McCord-Maughon ,
K. Staub ,
M.D. Levin ,
T. Parker ,
S.E. Fraser  and
S.R. Marder
M.E. Dickinson
Affiliation:
Biological Imaging Center, Pasadena, CA91125
D. McCord-Maughon
Affiliation:
Molecular Materials Resource Center, Beckman Institute, California Institute of Technology, Pasadena, CA91125.
K. Staub
Affiliation:
Molecular Materials Resource Center, Beckman Institute, California Institute of Technology, Pasadena, CA91125.
M.D. Levin
Affiliation:
Molecular Materials Resource Center, Beckman Institute, California Institute of Technology, Pasadena, CA91125.
T. Parker
Affiliation:
Molecular Materials Resource Center, Beckman Institute, California Institute of Technology, Pasadena, CA91125. Present Address: Departent of Chemistry, University of Arizona, Tuscon, AZ85721
S.E. Fraser
Affiliation:
Biological Imaging Center, Pasadena, CA91125
S.R. Marder
Affiliation:
Molecular Materials Resource Center, Beckman Institute, California Institute of Technology, Pasadena, CA91125. Present Address: Departent of Chemistry, University of Arizona, Tuscon, AZ85721
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Extract

One current limitation of two-photon technology is the lack of optimized fluorochromes for Two-Photon Laser Scanning Microscopy (TPLSM). Recently, Albota et al. (1998) have reported the design and synthesis of a number of bis(styryl)benzene derivatives that have large fluorescence quantum yields and greater two-photon absoption cross sections than any of fluorochromes that are currently used for TPLSM. As a first step in understanding how such molecules could be used in biological experiments, we have tagged a donor-πdonor molecule with biotin. We have successfully used this dye to detect quail nuclei in 2- 4 day old fixed embryos that have been reacted with QCPN, a quail specific monoclonal antibody. We have observed that this molecule gives a very intense signal, among the most intense signals we have been able to measure in our immunoassay. In particular, the brightness was about a factor of four higher than Rhodamine B-labelled streptavidin (containing 3.3 moles of dye per streptavidin tetramer).

Type
Multi Photon Excitation Microscopy: The Next Generation
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1062 - 1063
Copyright
Copyright © Microscopy Society of America

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References

Albota, M. et al. Design of Organic Molecules with Large Two-photon Absorption Cross Sections. Science 281,1653–1656.CrossRefGoogle Scholar