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All Models are Wrong an Overview of 3D Deconvolution Methods

Published online by Cambridge University Press:  02 July 2020

José-Angel Conchello ,
Joanne Markham  and
James G. McNally
José-Angel Conchello
Affiliation:
Institute for Biomédical Computing, St. Louis, MO, 63110
Joanne Markham
Affiliation:
Institute for Biomédical Computing, St. Louis, MO, 63110
James G. McNally
Affiliation:
Biology Department, Washington University, St. Louis, MO, 63110
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Three dimensional (3D)microscopy is a powerful toll for the visualization of biological specimens and processes. In 3D microscopy, a 3D image is collected by recording a series of two-dimensional (2D) images focusing the microscope at different planes through the specimen. Each 2D optical slice in this through focus series contains the in-focus information plus contributions from out-of-focus structures that obscure the image and reduce its contrast. There are two complementary approaches to reduce or ameliorate the effects of the out-of-focus contributions, optical and computational. In the optical approach a microscope is used that avoids collecting out-of-focus light, such as a confocal microscope (see and references therein), a two-photon or three-photon fluorescence excitation microscope, or atwo-sided microscope. In the computational approach, the through-focus series is processed in a computer using any of a number of debluring algorithms to reduce or ameliorate the out-of-focus contributions. In the past two decades, several methods for debluring microscopic images have been developed whose common aim is to undo the degradations introduced by the process of image formation and recording

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Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 375 - 376
Copyright
Copyright © Microscopy Society of America 1997

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