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Adaptation Of Environmental Transmission Electron Microscopy (ETEM) And Electron Energy Loss Spectrometry (EELS) For Studies Of Microbiologically Influenced Corrosion

Published online by Cambridge University Press:  02 July 2020

Robert K. Pope ,
Tyrone L. Daulton ,
Richard I. Ray  and
Brenda J. Little
Robert K. Pope
Affiliation:
Oceanography Division, Naval Research Laboratory, Stennis Space Center, MS39529
Tyrone L. Daulton
Affiliation:
Marine Geosciences Division, Naval Research Laboratory, Stennis Space Center, MS39529
Richard I. Ray
Affiliation:
Oceanography Division, Naval Research Laboratory, Stennis Space Center, MS39529
Brenda J. Little
Affiliation:
Oceanography Division, Naval Research Laboratory, Stennis Space Center, MS39529
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Extract

Microbiologically influenced corrosion (MIC) is of wide concern in marine and non-marine environments. Biofilms and corrosion products associated with microorganisms cause numerous problems in aqueous environments, such as increased fluid frictional resistance, reduced heat transfer, and many types of corrosion, all of which can lead to failure of materials. Corrosion of metals has been extensively examined using TEM, but examination of MIC with TEM has only just begun (Blunn, 1986; Chio, 1996). Previous studies examining microbial colonization of copper surfaces and distribution throughout corrosion products demonstrate copper immobilization by bacterial biofilms (Blunn, 1987). In the current study, Pseudomonasputida attachment to corroding iron particles was examined in a sealed environmental cell in a JEOL 3010 scanning transmission electron microscope (STEM).

Iron filings were produced from carbon steel (C1010) using 600 grit sandpaper, collected with a teflon coated magnet, degreased in acetone and sterilized in ethanol. Filings were incubated in distilled water until corrosion was visible under a dissecting microscope.

Type
Biological Microanalysis
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 904 - 905
Copyright
Copyright © Microscopy Society of America

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References

References:

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6. This work was performed under Program Element 0601153N, NRL Contribution Number NRL/BA/7303-99-0002. Robert K. Pope was supported by a CORE/NRL Postdoctoral FellowshipGoogle Scholar