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Short-Term Response of Mitomycin C on the Human Rectus Muscle Following Strabismus Surgery: Histological, Ultrastructural, and Biomechanical Evaluation

Published online by Cambridge University Press:  04 February 2013

Samjin Choi
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Orthodontics, Kyung Hee University, Seoul, Korea
Youjin Cheong
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea
Jae-Ho Shin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Kyung A Kim
Affiliation:
Department of Orthodontics, Kyung Hee University, Seoul, Korea
Jae Beum Bang
Affiliation:
Department of Dental Education, Kyung Hee University, Seoul, Korea
Kyung-Hyun Jin
Affiliation:
Department of Ophthalmology, Kyung Hee University, Seoul, Korea
Hun-Kuk Park*
Affiliation:
Department of Biomedical Engineering & Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea Department of Medical Engineering, Kyung Hee University, Seoul, Korea
*
*Corresponding author. E-mail: sigmoidus@khu.ac.kr
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Abstract

This study investigated the inflammatory effect of intraoperative mitomycin C (MMC) on adhesion reformation in human rectus muscles. Ten consecutive patients who underwent medial rectus resection had their postoperative rectus muscles divided into two groups: control group (n = 10) and MMC group (n = 10). In the MMC group, the muscle was soaked for 2 min with MMC, prepared as a 0.2 mg/mL (0.02%) solution. The 0.02% MMC reactions were examined using histological analysis with hematoxylin-eosin (inflammatory response) and Masson's trichrome (collagen fibrils), immunoreactivities of cyclooxygenase-II (inflammatory response), and collagen type I and III, scanning electron microscopy analysis to quantify the diameter and D-periodicity of collagen fibrils, and atomic force microscopy analysis to quantify the diameter, D-periodicity, and adhesion force of collagen fibrils. The rectus muscles treated with 0.02% MMC showed a significantly increased inflammatory response (p < 0.05), increased collagen density (p < 0.0001), increased fibril diameter (p < 0.001 or p < 0.05), and decreased fibril adhesion force (p < 0.005) compared to the rectus muscles in the control group. MMC simultaneously caused an inflammatory response as well as nanostructural and biomechanical property changes in the collagen fibril network.

Type
Biological Applications
Copyright
Copyright © Microscopy Society of America 2013

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