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Morphological Changes in the Myotendinous Junction of mdx Mice

Published online by Cambridge University Press:  11 August 2021

Giovana Zerbo Martinez
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Bruna Aléxia Cristofoletti Grillo
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Lara Caetano Rocha
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Carolina dos Santos Jacob
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Jurandyr Pimentel Neto
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
André Neri Tomiate
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Gabriela Klein Barbosa
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
Ii-sei Watanabe
Affiliation:
Department of Anatomy, Institute of Biomedical Sciences III, University of São Paulo (USP), São Paulo, SP, Brazil
Adriano Polican Ciena*
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), São Paulo State University (UNESP), Institute of Biosciences (IB), Avenue 24A, n. 1515, Rio Claro, SP 13506-900, Brazil
*
*Correspondent author: Adriano Polican Ciena, E-mail: adriano.ciena@unesp.br

Abstract

The myotendinous junction (MTJ) is the interface between muscle and tendon, and it is the main area of force transmission of the locomotor apparatus. Dystrophic processes promote pathological injury which affects the skeletal muscle and can influence the morphology of the MTJ. This study aimed to investigate the adaptations in MTJ morphology of mdx mice in the tibialis anterior muscle. Male mice (n = 24) were divided into Control—C57bl/10 and mdx—C57bl/10mdx (Duchenne muscular dystrophy experimental model). In the mdx group, centralized nuclei with a large area and greater deposition of type III collagen (fibrosis) were observed. Also, shorter sarcomeres and sarcoplasmatic projections of MTJ were observed. We concluded that the adaptations in mdx mice demonstrated extensive impairment in the MTJ region with reduced ultrastructures.

Type
Micrographia
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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