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Migratory Activities and Stemness Properties of Rodlet Cells

Part of: Micrographia Collection

Published online by Cambridge University Press:  21 August 2020

Hanan H. Abd-Elhafeez Open the ORCID record for Hanan H. Abd-Elhafeez [Opens in a new window] ,
Alaa S. Abou-Elhamd Open the ORCID record for Alaa S. Abou-Elhamd [Opens in a new window] ,
Walied Abdo Open the ORCID record for Walied Abdo [Opens in a new window]  and
Soha A. Soliman Open the ORCID record for Soha A. Soliman [Opens in a new window]
Hanan H. Abd-Elhafeez*
Affiliation:
Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut71526, Egypt
Alaa S. Abou-Elhamd
Affiliation:
Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut71526, Egypt
Walied Abdo
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Kafr El Sheikh University, Kafr El Sheikh33516, Egypt
Soha A. Soliman
Affiliation:
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena83523, Egypt
*
*Author for correspondence: Hanan H. Abd-Elhafeez, E-mail: hhmmzz91@gmail.com.
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Abstract

The current study aimed to characterize different stages of rodlet cells using light microscopy, immunohistochemistry, and transmission electron microscopy. Granular rodlet cells have a distinct granular cytoplasm. Transitional rodlet cells had distinct capsules, and immature granules. Mature rodlet cells were pear-shaped and had elongated granules. Ruptured rodlet cells had a granular cytoplasm. The affinity of rodlet cells for different histochemical techniques was detected. Immunohistochemical analysis of rodlet cells for stem cell markers such as CD117, CD34, proliferation marker, proliferating cell nuclear antigen (PCNA), endopeptidase activity; matrix metalloproteinase-9 (MPP-9) and the angiogenic factor; vascular endothelial growth factor (VEGF) was investigated. All stages of rodlet cells were expressed CD117. However, the ruptured stage was CD117-negative. The granular, transitional, and mature stages had strong CD34 immunoaffinity, while the ruptured rodlet cells were CD34-negative. The most potent immunoreactivity for PCNA was the granular rodlet cells. The transitional cells exhibited less immunoreactivity, while mature rodlet cells had no immunoaffinity for PCNA. All stages of rodlet cells had high enzyme activity as indicated by Acridine orange and exhibited strong MPP-9 immunoaffinity. VEGF is mostly expressed by granular, transitional, and mature rodlet cells. In conclusion, rodlet cells relatively had stemness properties, endopeptidase activity, express a proliferation marker, and angiogenic factors. We suggest a potential role of rodlet cells in immune defense.

Keywords

CD117 and CD 34MPP-9PCNArodlet cellsVEGF
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 26 , Issue 5 , October 2020 , pp. 1035 - 1052
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Copyright
Copyright © Microscopy Society of America 2020

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