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High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2

Published online by Cambridge University Press:  02 July 2020

D.R. Keene
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
N.L. Charbonneau
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
B.J. Dzamba
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
D.P. Reinhardt
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
C.C. Ridgway
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
R.N. Ono
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
L.Y. Sakai
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201

Extract

Immunolocalization studies of neonate and older human tissues have previously demonstrated that fibrillin-1 is a component of elastin and non-elastin associated microfibrils. In sections taken from fixed, dehydrated and embedded tissue, microfibrils appear in the transmission electron microscope as hollow rods, 6-8 nm in diameter. When isolated from tissue by homogenization and observed following negative staining or rotary shadowing, microfibrils appear as beaded strings with a degree of extendibility. Recently, a closely related glycoprotein, fibrillin-2, has been described, which is expressed in early fetal development, prior to fibrillin-1, but then disappears in most tissues just prior to birth. We demonstrate here the characterization monoclonal antibodies specific for fibrillin-2. The antibodies are shown by ELISA and immunoblots to be fibrillin-2 specific. The matrix from a variety of tissues, including aorta, tendon, and eye are shown to contain fibrillin-2 by immunofluorescence in a pattern similar to that of fibrillin-1.

Type
Developmental Biology
Copyright
Copyright © Microscopy Society of America

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References

1. Sakai, L.Y., Keene, D.R., and Engvall, E. (1986a) Fibrillin, a new 350 kd glycoprotein, is a component of extracellular microfibrils. J. Cell Biol., 103:24992509.CrossRefGoogle Scholar
2.Maddox, B. K., Sakai, L.Y., Keene, D. R., and Glanville, R. W.: Connective tissue microfibrils: Isolation and characterization of three large pepsin resistant domains of fibrillin. J. Biol. Chem., 264: 2138121385, 1989.Google ScholarPubMed
3.Zhang, H., Apfelroth, S.D., Hu, W., Davis, E.C., Sanguineti, C, Bonadio, J., Mecham, R.P., Ramirez, F. (1994) Structure and expression of fibrillin-2, a novel microfibrillar component preferentially located in elastic matrices. J. Cell Biol., 124:855863.CrossRefGoogle ScholarPubMed

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High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2
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High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2
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High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2
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