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Defective Histone H3K27 Trimethylation Modification in Embryos Derived from Heated Mouse Sperm

Published online by Cambridge University Press:  09 May 2012

Shi-Bin Chao
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Lei Chen
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Jian-Chun Li
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China
Xiang-Hong Ou
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Xiao-Jin Huang
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China
Shu Wen
Department of Obstetrics and Gynecology, One Baylor Plaza, Baylor College of Medicine, Houston, TX, USA
Qing-Yuan Sun
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Guo-Lan Gao*
Center of Reproductive Medicine, The First Affiliated Hospital of NanChang University, NanChang, China Department of Obstetrics and Gynecology, Aviation General Hospital of China, Beijing, China
Corresponding author. E-mail:
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The mouse sperm genome is resistant to in vitro heat treatment, and embryos derived from heated sperm can support full-term embryonic development, but the blastocyst rate and implantation rate are lower compared to embryos derived from fresh sperm. In the present study, the patterns of DNA methylation, histone H4K12 (ACH4K12) acetylation, H3K9 trimethylation (H3K9-TriM), and H3K27 trimethylation (H3K27-TriM) in preimplantation embryos derived from 65°C-heated sperm were investigated. Although no evident changes in global DNA methyaltion, histone H4K12 (ACH4K12) acetylation, and H3K9 trimethylation (H3K9-TriM) were found, significantly lower levels of H3K27-TriM, which was thought to be one of the reasons for low efficiency of mouse cloning, were found in the inner cell mass of heated-sperm derived blastocysts. Thus, defective modification of H3K27-TriM might contribute to compromised development of embryos derived from heated sperm.

Biological Applications
Copyright © Microscopy Society of America 2012

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