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Atomic Force Microscopy Studies on DNA Structural Changes Induced by Vincristine Sulfate and Aspirin

Published online by Cambridge University Press:  17 March 2004

Yi Zhu
Affiliation:
Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China
Hu Zeng
Affiliation:
Model Animal Research Center, Nanjing University, Nanjing 210093, P.R. China
Jianming Xie
Affiliation:
Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China
Long Ba
Affiliation:
Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China
Xiang Gao
Affiliation:
Model Animal Research Center, Nanjing University, Nanjing 210093, P.R. China
Zuhong Lu
Affiliation:
Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China
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Abstract

We report that atomic force microscopy (AFM) studies on structural variations of a linear plasmid DNA interact with various concentrations of vincristine sulfate and aspirin. The different binding images show that vincrinstine sulfate binding DNA chains caused some loops and cleavages of the DNA fragments, whereas aspirin interaction caused the width changes and conformational transition of the DNA fragments. Two different DNA structural alternations could be explained by the different mechanisms of the interactions with these two components. Our work indicates that the AFM is a powerful tool in studying the interaction between DNA and small molecules.

Type
Biological Applications
Copyright
© 2004 Microscopy Society of America

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References

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