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An Improved Cryogen for Plunge Freezing

Published online by Cambridge University Press:  16 September 2008

William F. Tivol*
Affiliation:
California Institute of Technology, Broad Center, MC 114-96, 1201 E. California Blvd., Pasadena, CA 91125, USA
Ariane Briegel
Affiliation:
California Institute of Technology, Broad Center, MC 114-96, 1201 E. California Blvd., Pasadena, CA 91125, USA
Grant J. Jensen
Affiliation:
California Institute of Technology, Broad Center, MC 114-96, 1201 E. California Blvd., Pasadena, CA 91125, USA
*
Corresponding author. E-mail: tivol@caltech.edu

Abstract

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

Type
Biological Applications
Copyright
Copyright © Microscopy Society of America 2008

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References

Briegel, A., Ding, H.J., Li, Z., Werner, J., Gitai, Z., Dias, D.P., Jensen, R.B. & Jensen, G. (2008). Location and architecture of the Caulobacter crescentus chemoreceptor array. Mol Microbiol 69, 3041.CrossRefGoogle ScholarPubMed
Brüggeller, P. & Mayer, E. (1980). Complete vitrification in pure liquid water and dilute aqueous solutions. Nature 288, 569571.CrossRefGoogle Scholar
Griffith, C.A., Penteado, P., Rannou, P., Brown, R., Boudon, V., Baines, K.H., Clark, R., Drossart, P., Buratti, B., Nicholson, P., McKay, C.P., Coustenis, A., Negrao, A. & Jaumann, R. (2006). Evidence for a polar ethane cloud on Titan. Science 313, 16201622.CrossRefGoogle ScholarPubMed
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