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Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil

Published online by Cambridge University Press:  22 February 2013

Thais G. Corrêa Sholl
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil
Jailson Fulgencio de Moura
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil Programa de Pós Graduação em Saúde Pública e Meio Ambiente, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz—ENSP/FIOCRUZ, Brazil
Paulo Henrique Ott
Affiliation:
Universidade Estadual do Rio Grande do Sul—UERGS–Unidade Litoral Norte, Osório, Laboratório de Biologia da Conservação de Aves e Mamíferos Aquáticos (LABCAM); Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Avenida Tramandaí, Imbé, R5, 95625-000, 90440-150, Brazil
Cibele R. Bonvicino
Affiliation:
Divisão de Genética, Instituto Nacional de Câncer, Rua André Cavalcante 37, 4o andar, Centro, RJ, 20231-050, Brazil and Laboratório de Biologia e Parasitologia de Mamíferos Reservatórios, Instituto Oswaldo Cruz/FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil
Estéfane C. Reis
Affiliation:
Laboratório de Genética Marinha (LGMar), Departamento de Genética, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524-PHLC-Sala 205, Maracanã, Rio de Janeiro, RJ, 20550-013, Brazil
Davi Castro Tavares
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil PPG Ecologia e Recursos Naturais, Universidade Estadual do Norte Fluminense Darcy Ribeiro–UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ 28013-602 Brazil
Salvatore Siciliano
Affiliation:
Fundação Oswaldo Cruz—FIOCRUZ, Escola Nacional de Saúde Pública—ENSP, Departamento de Endemias, Grupo de Estudos de Mamíferos Marinhos da Região dos Lagos—GEMM-Lagos, Rua Leopoldo Bulhões, 1480–6° andar, Manguinhos, Rio de Janeiro, RJ 21041-210, Brazil
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Abstract

Carcasses of whales provide much valuable information on their natural history. However, some specimens cannot be identified in the field due to the advanced state of decomposition. In this study, the DNA was extracted and the mitochondrial cytochrome b gene was amplified by polymerase chain reaction and sequenced for four carcasses of possible mysticeti (GEMM: 075, 088, 135 and GEMARS: 1302). A blast search using the nucleotide–nucleotide basic local alignment (blastn) search tool was conducted using the generated sequences. Samples GEMM 075 and GEMARS 1302 showed 98% identity to one sequence of Balaenoptera acutorostrata. Samples GEMM 088 and GEMM 135 showed 99% identity to sequences from Balaenoptera edeni and Megaptera novaeangliae, respectively. A neighbour-joining tree was generated using sequences from GenBank from all species of balaenopterid that occur on the coast of Brazil. The results showed that all carcasses analysed were correspondent to species from the family Balaenopteridae already recorded in Brazil.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013

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