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Palicella, a new genus of lichenized fungi and its phylogenetic position within Lecanoraceae

Published online by Cambridge University Press:  09 July 2014

Pamela RODRIGUEZ FLAKUS  and
Christian PRINTZEN
Pamela RODRIGUEZ FLAKUS
Affiliation:
Department of Botany and Molecular Evolution, Senckenberg Research Institute, Senckenberganlage 25, D-60325, Frankfurt am Main, Germany. Email: pamela.rodriguez@senckenberg.de
Christian PRINTZEN
Affiliation:
Department of Botany and Molecular Evolution, Senckenberg Research Institute, Senckenberganlage 25, D-60325, Frankfurt am Main, Germany. Email: pamela.rodriguez@senckenberg.de
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Abstract

Non-saxicolous lecideoid lichens form a diverse and polyphyletic group of species with a worldwide distribution. The phylogenetic relationships of the taxa and major clades are still largely unresolved. Here we introduce a new genus Palicella Rodr. Flakus & Printzen for Lecidea glaucopa Hook. f. & Tayl. and two closely related species. Our molecular phylogenetic results, based on maximum likelihood and Bayesian phylogenetic trees, and a combined dataset of five gene loci (ITS, nrLSU, mrSSU, RPB1 and RPB2), indicate that Palicella forms a monophyletic clade sister to Lecanora symmicta and Pyrrhospora quernea. The new genus is characterized by biatorine apothecia, which are often darkened (by the pigment Cinereorufa-green), an exciple of radiating, narrow hyphae with strongly elongate lumina, a hymenium with branched and sparsely anastomosed paraphyses, an ascus with a broad axial body surrounded by a distinct darker staining layer, and narrowly ellipsoid ascospores. Atranorin is present in all, usnic acid in two and pannarin in one species of the genus. Three new combinations are proposed: Palicella filamentosa (Stirt.) Rodr. Flakus & Printzen, P. glaucopa (Hook. f. & Taylor) Rodr. Flakus & Printzen, and P. schizochromatica (Pérez-Ortega, T. Sprib. & Printzen) Rodr. Flakus & Printzen. The molecular phylogenetic analysis confirms that Lecidea hercynica Hauck & Schmull is a synonym of P. filamentosa. An identification key to the species is provided.

Keywords

combined analysisLecanoraLecanoralesLecidealecideoid lichenstaxonomy
Type
Articles
Information
The Lichenologist , Volume 46 , Issue 4 , July 2014 , pp. 535 - 552
Copyright
Copyright © British Lichen Society 2014 

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References

Acharius, E. (1810) Lichenographia Universalis. Göttingen: J. F. Danckwerts.Google Scholar
Acharius, E. (1814) Synopsis Methodica Lichenum. Lund: Svanborg & Soc.Google Scholar
Andersen, H. L. & Ekman, S. (2005) Disintegration of the Micareaceae (lichenized Ascomycota): a molecular phylogeny based on mitochondrial rDNA sequences. Mycological Research 109: 21–30.Google Scholar
Arup, U. & Grube, M. (1998) Molecular systematics of Lecanora subgenus Placodium . Lichenologist 30: 415–425.CrossRefGoogle Scholar
Arup, U. & Grube, M. (2000) Is Rhizoplaca (Lecanorales, lichenized Ascomycota) a monophyletic genus? Canadian Journal of Botany 78: 318–327.CrossRefGoogle Scholar
Brako, L. (1991) Phyllopsora (Bacidiaceae). Flora Neotropica 55: 1–66.Google Scholar
Buschbom, J. & Mueller, G. (2004) Resolving evolutionary relationships in the lichen-forming genus Porpidia and related allies (Porpidiaceae, Ascomycota). Molecular Phylogenetics and Evolution 32: 66–82.Google Scholar
Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540–552.CrossRefGoogle ScholarPubMed
Choisy, M. (1929) Genres nouveaux pour la lichénologie dans le groupe des Lécanoracées. Bulletin de la Société Botanique de France 76: 521–527.CrossRefGoogle Scholar
Coppins, B. J. (1983) A taxonomic study of the lichen genus Micarea in Europe. Bulletin of the British Museum (Natural History), Botany 11: 17–214.Google Scholar
Culberson, C. F. & Kristinsson, H. (1970) A standardized method for the identification of lichen products. Journal of Chromatography 46: 85–93.Google Scholar
Czarnota, P. (2007) The lichen genus Micarea (Lecanorales, Ascomycota) in Poland. Polish Botanical Studies 23: 1–199.Google Scholar
Edgar, R. C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5: 113.Google Scholar
Ekman, S. (1997) The genus Cliostomum revisited. Symbolae Botanicae Upsalienses 32: 17–28.Google Scholar
Ekman, S. (2004) Cliostomum. In Lichen Flora of the Greater Sonoran Desert Region, Vol. 2 (Nash, T. H. III, Ryan, B. D., Diederich, P., Gries, C. & Bungartz, F., eds): 64–65. Tempe, Arizona: Lichens Unlimited, Arizona State University.Google Scholar
Elix, J. A. (2009) Ramboldia. Flora of Australia 57: 19–31.Google Scholar
Feige, G. B., Lumbsch, H. T., Huneck, S. & Elix, J. A. (1993) Identification of lichen substances by a standardized high-performace liquid chromatographic method for lichen products. Journal of Chromatography 646: 417–427.Google Scholar
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118.Google Scholar
Hafellner, J. (1984) Studien in Richtung einer natürlicheren Gliederung der Sammelfamilien Lecanoraceae und Lecideaceae . Beiheft zur Nova Hedwigia 79: 241–371.Google Scholar
Hafellner, J. (1993) Die Gattung Pyrrhospora in Europa. Herzogia 9: 725–747.CrossRefGoogle Scholar
Hedlund, T. (1892) Kritische Bemerkungen über einige Arten der Flechtengattungen Lecanora (Ach.), Lecidea (Ach.) und Micarea (Fr.). Bihang till kungliga svenska Vetenskaps-Akademiens Handlingar 18: 1–14.Google Scholar
Hertel, H. (1967) Revision einiger calciphiler Formenkreise der Flechtengattung Lecidea . Beiheft zur Nova Hedwigia 24: 1–174.Google Scholar
Hertel, H. (1983) Über einige aus Lecidea und Melanolecia (Ascomycetes lichenisati) auszuschließende Arten. Mitteilungen der Botanischen Staatssammlung München 19: 441–447.Google Scholar
Hertel, H. (1984) Über saxicole, lecideoide Flechten der Subantarktis. Beiheft zur Nova Hedwigia 79: 399–499.Google Scholar
Hertel, H. (1995) Schlüssel für die Arten der Flechtenfamilie Lecideaceae in Europa. Bibliotheca Lichenologica 58: 137–180.Google Scholar
Hertel, H. (1997) On the genus Lecidea (Lecanorales) in southern Chile and Argentina. Symbolae Botanicae Upsalienses 32: 95–111.Google Scholar
Hertel, H. (2006) World distribution of species of Lecidea (Lecanorales) occurring in Central Europe. In Central European Lichens – Diversity and Threat (Lackovičová, A., Guttová, A., Lisická, E. & Lizoň, P., eds): 19–73. Ithaca: Mycotaxon Ltd.Google Scholar
Hertel, H. & Printzen, C. (2004) Lecidea. In Lichen Flora of the Greater Sonoran Desert Region, Vol. 2 (Nash, T. H. III, Ryan, B. D., Diederich, P., Gries, C. & Bungartz, F., eds): 287–309. Tempe, Arizona: Lichens Unlimited, Arizona State University.Google Scholar
Hooker, J. D. & Taylor, T. (1844) Lichenes Antarctici. London Journal of Botany 3: 634–658.Google Scholar
Huelsenbeck, J. P. & Ronquist, F. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.Google Scholar
Kalb, K. (1984) Lichenes Neotropici, Fascicle 8 (Nos. 301–350): 1–16. Neumarkt/OPf.Google Scholar
Kalb, K., Staiger, B., Elix, J. A., Lange, U. & Lumbsch, H. T. (2008) A new circumscription of the genus Ramboldia (Lecanoraceae, Ascomycota) based on morphological and molecular evidence. Nova Hedwigia 86: 23–42.Google Scholar
Kalb, K., Rivas Plata, E., Lücking, R. & Lumbsch, H. T. (2011) The phylogenetic position of Malmidea, a new genus for the Lecidea piperis and Lecanora granifera-groups (Lecanorales, Malmideaceae) inferred from nuclear and mitochondrial ribosomal DNA sequences with special reference to Thai species. Bibliotheca Lichenologica 106: 143–168.Google Scholar
Kantvilas, G. & Elix, J. A. (1994) Ramboldia, a new genus in the lichen family Lecanoraceae . Bryologist 97: 296–304.CrossRefGoogle Scholar
Kantvilas, G. & Elix, J. A. (2013) The lichen genus Lecidella (Lecanoraceae), with special reference to the Tasmanian species. Muelleria 31: 31–47.Google Scholar
Knoph, J. G. (1990) Untersuchungen an gesteinsbewohnenden xanthonhaltigen Sippen der Flechtengattung Lecidella (Lecanoraceae, Lecanorales) unter besonderer Berücksichtigung von außereuropäischen Proben exklusive Amerika. Bibliotheca Lichenologica 36: 1–183.Google Scholar
Knoph, J. G. & Leuckert, C. (2004) Lecidella. In Lichen Flora of the Greater Sonoran Desert Region, Vol. 2 (Nash, T. H. III, Ryan, B. D., Diederich, P., Gries, C. & Bungartz, F., eds): 309–320. Tempe, Arizona: Lichens Unlimited, Arizona State University.Google Scholar
Lanfear, R., Calcott, B., Ho, S. Y. W. & Guidon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29: 1695–1701.CrossRefGoogle ScholarPubMed
Liu, Y. J., Whelen, S. & Hall, B. D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808.Google Scholar
Matheny, P. B., Liu, Y. J., Ammirati, J. F. & Hall, B. D. (2002) Using RPB1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). American Journal of Botany 89: 688–698.Google Scholar
Meyer, B. & Printzen, C. (2000) Proposal for a standardized nomenclature and characterization of insoluble lichen pigments. Lichenologist 32: 571–583.Google Scholar
Miądlikowska, J., Kauff, F., Hofstetter, V., Fraker, E., Grube, M., Hafellner, J., Reeb, V., Hodkinson, B. P., Kukwa, M., Lücking, R., et al. (2006) New insights into classification and evolution of the Lecanoromycetes (Pezizomycotina, Ascomycota) from phylogenetic analyses of three ribosomal RNA and two protein-coding genes. Mycologia 98: 1088–1103.Google Scholar
Nylander, W. (1888) Lichenes Fuegiae et Patagoniae. Paris: Impr. de A. Héloin et E. Charles.Google Scholar
Orange, A., James, P. W. & White, F. J. (2001) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.Google Scholar
Palice, Z., Printzen, C., Spribille, T. & Elix, J. A. (2011) Notes on the synonyms of Lecanora filamentosa . Graphis Scripta 23: 1–7.Google Scholar
Pérez-Ortega, S., Spribille, T., Palice, Z., Elix, J. A. & Printzen, C. (2010) A molecular phylogeny of the Lecanora varia group, including a new species from western North America. Mycological Progress 9: 523–535.Google Scholar
Printzen, C. (1995) Die Flechtengattung Biatora in Europa. Bibliotheca Lichenologica 60: 1–275.Google Scholar
Printzen, C. (2001) Corticolous and lignicolous species of Lecanora (Lecanoraceae, Lecanorales) with usnic or isousnic acid in the Sonoran Desert Region. Bryologist 104: 382–409.Google Scholar
Printzen, C. & Kantvilas, G. (2004) Hertelidea, genus novum Stereocaulacearum (Ascomycetes lichenisati). Bibliotheca Lichenologica 88: 539–553.Google Scholar
Printzen, C. & May, P. (2002) Lecanora ramulicola (Lecanoraceae, Lecanorales), an overlooked lichen species from the Lecanora symmicta group. Bryologist 105: 63–69.CrossRefGoogle Scholar
Printzen, C. & Tønsberg, T. (1999) The lichen genus Biatora in northwestern North America. Bryologist 102: 692–713.Google Scholar
Rambold, G. (1989) A monograph of the saxicolous lecideoid lichens of Australia (excl. Tasmania). Bibliotheca Lichenologica 34: 1–345.Google Scholar
Räsänen, V. (1932) Zur Kenntnis der Flechtenflora Feuerlands. Annales Botanici Societatis Zoologicae-Botanicae Fennicae Vanamo 2: 1–65.Google Scholar
Rehner, S. A. & Samuels, G. J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98: 625–634.CrossRefGoogle Scholar
Schmitt, I., Lumbsch, H. T. & Søchting, U. (2003) Phylogeny of the lichen genus Placopsis and its allies based on Bayesian analyses of nuclear and mitochondrial sequences. Mycologia 95: 827–835.CrossRefGoogle ScholarPubMed
Schmull, M. & Hauck, M. (2005) Lecidea hercynica, a new montane epiphytic lichen from Germany. Lichenologist 37: 485–489.Google Scholar
Schmull, M., Miądlikowska, J., Pelzer, M., Stocker-Wörgötter, E., Hofstetter, V., Fraker, E., Hodkinson, B. P., Reeb, V., Kukwa, M., Lumbsch, H. T., et al. (2011) Phylogenetic affiliations of members of the heterogeneous lichen-forming fungi of the genus Lecidea sensu Zahlbruckner (Lecanoromycetes, Ascomycota). Mycologia 103: 983–1003.Google Scholar
Silvestro, D. & Michalak, I. (2012) RaxmlGUI: a graphical front-end for RAxML. Organisms Diversity and Evolution 12: 335–337.Google Scholar
Śliwa, L. & Wetmore, C. M. (2000) Notes on the Lecanora varia group in North America. Bryologist 103: 475–492.Google Scholar
Spribille, T., Goffinet, B., Muggia, L., Obermayer, W. & Mayrhofer, H. (2011) Molecular support for the recognition of the Mycoblastus fucatus group as the new genus Violella (Tephromelataceae, Lecanorales). Lichenologist 43: 445–466.Google Scholar
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.CrossRefGoogle ScholarPubMed
Stiller, J. W. & Hall, B. D. (1997) The origin of red algae: implications for plastid evolution. Proceedings of the National Academy of Sciences of the United States of America 94: 4520–4525.CrossRefGoogle ScholarPubMed
Stirton, J. (1879) New and rare Scottish lichens. Scottish Naturalist 5: 217–221.Google Scholar
Timdal, E. (1991) A monograph of the genus Toninia (Lecideaceae, Ascomycetes). Opera Botanica 110: 1–137.Google Scholar
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species Journal of Bacteriology 172: 4238–4246.Google Scholar
Vobis, G. (1980) Bau und Entwicklung der Flechten-Pycnidien und ihrer Conidien. Bibliotheca Lichenologica 14: 1–141.Google Scholar
White, T. J., Bruns, T., Lee, S. & Taylor, J. W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J., eds): 315–322. New York: Academic Press.Google Scholar
Zahlbruckner, A. (1925) Catalogus Lichenum Universalis. Vol. 3. Leipzig: Bornträger.Google Scholar
Zahlbruckner, A. (1928) Catalogus Lichenum Universalis. Vol. 5. Leipzig: Bornträger.Google Scholar
Zahlbruckner, A. (1940) Catalogus Lichenum Universalis. Vol. 10. Leipzig: Bornträger.Google Scholar
Zhou, S. & Stanosz, G. R. (2001) Primers for amplification of mtSSU rDNA, and a phylogenetic study of Botryosphaeria and associated anamorphic fungi. Mycological Research 105: 1033–1044.Google Scholar
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31: 511–516.Google Scholar