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New polymorphic mitochondrial markers for sponge phylogeography

Published online by Cambridge University Press:  01 February 2011

Cintia P.J. Rua ,
Carla Zilberberg  and
Antonio M. Solé-Cava
Cintia P.J. Rua
Affiliation:
Departamento de Genética, Universidade Federal do Rio de Janeiro, Brazil
Carla Zilberberg
Affiliation:
Departamento de Genética, Universidade Federal do Rio de Janeiro, Brazil Departamento de Zoologia, Universidade Federal do Rio de Janeiro, Brazil
Antonio M. Solé-Cava*
Affiliation:
Departamento de Genética, Universidade Federal do Rio de Janeiro, Brazil
*
Correspondence should be addressed to: A.M. Solé-Cava, Laboratório de Biodiversidade Molecular, A2-098, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941-590–Rio de Janeiro, RJ, Brazil email: sole@biologia.ufrj.br
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Abstract

Phylogeography and population genetic studies in the Porifera have been limited by the lack of available polymorphic DNA markers. In this paper, we tested four new mitochondrial markers in nine demosponge species from a wide taxonomic range: partial sequences of the ATP synthase 6 (ATP6) and the cytochrome oxidase 2 (CO2) genes and two spacers: one located between ATP6 and CO2 and the other between the NADH dehydrogenase subunit 5 (ND5) and the small subunit ribosomal RNA (rns) genes. The new markers presented levels of nucleotide diversity up to 2.4 times higher (π = 0.015 for CO2) than those observed for the most commonly used mitochondrial marker in sponges, the cytochrome oxidase 1 gene (π = 0.006), making them suitable for alpha-level systematics, phylogeography and population genetics studies. 

Keywords

DNA markersPoriferapopulation genetics
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 91 , Issue 5 , August 2011 , pp. 1015 - 1022
Copyright
Copyright © Marine Biological Association of the United Kingdom 2011

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