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Multiplex fluorimetric assays for monitoring algal toxins

Published online by Cambridge University Press:  02 December 2008

Carmen K.M. Mak ,
Patrick K.K. Yeung ,
Alvin C.M. Kwok ,
Y.H. Wong  and
Joseph T.Y. Wong
Carmen K.M. Mak
Affiliation:
Biology Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Patrick K.K. Yeung
Affiliation:
Biology Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Alvin C.M. Kwok
Affiliation:
Biology Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Y.H. Wong
Affiliation:
Biotechnology Research Institute and Biochemistry Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Joseph T.Y. Wong*
Affiliation:
Biology Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
*
Correspondence should be addressed to: J.T.Y. Wong, Biology Department, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong email: botin@ust.hk
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Abstract

Most known algal toxins act on ion channels either directly or indirectly, resulting in a change in intracellular ion concentrations when administered to targeted cells. The present project developed the working conditions for the use of fluorescent dyes in monitoring changes in membrane potential, intracellular calcium, and intracellular sodium levels in mammalian cell lines. Using these conditions, we were able to demonstrate specific changes in fluorescent signals in response to several purified toxins. We were also able to generate algal extracts which, when administered to the developed fluorimetric assays, were able to elicit different pattern of changes in membrane potential, intracellular calcium, and intracellular sodium levels. The differential pattern of responses induced by the different algal toxins in the three fluorimetric assays serve as a proof of concept for the use of multiplex fluorimetric assays in the laboratory monitoring of algal toxins.

Keywords

multiplex fluorimetric assaysmonitoringalgal toxins
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 89 , Issue 2 , March 2009 , pp. 287 - 293
Copyright
Copyright © Marine Biological Association of the United Kingdom 2008

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