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Marking octopus (Octopus vulgaris) paralarvae statoliths with alizarin complexone

Published online by Cambridge University Press:  01 June 2000

L. Fuentes
Affiliation:
Instituto Español de Oceanografía, Centro Costero de Vigo, Cabo Estay, Canido, PO Box 1552, 36280 Vigo, Spain
J. Iglesias
Affiliation:
Instituto Español de Oceanografía, Centro Costero de Vigo, Cabo Estay, Canido, PO Box 1552, 36280 Vigo, Spain
C. Moxica
Affiliation:
Instituto Español de Oceanografía, Centro Costero de Vigo, Cabo Estay, Canido, PO Box 1552, 36280 Vigo, Spain

Abstract

It is possible to mark octopus (Octopus vulgaris) paralarvae on a massive scale (up to 3500 paralarvae l−1) with 100% marking reliability using alizarin complexone (ALC). The whole statolith is marked and the best results obtained were with concentrations of 60-300 mg l−1 and bath duration of 3-24 h. Taking into account the quality of marking, mortality and ALC costs suggest using from 60 to 200 mg l−1 for 3-6 h. The only treatments causing significant mortalities were 300 mg l−1 over 6 and 24 h.

Type
SHORT COMMUNICATION
Copyright
2000 Marine Biological Association of the United Kingdom

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