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Graphene/chitosan-functionalized iron oxide nanoparticles for biomedical applications

Published online by Cambridge University Press:  20 September 2019

Suresh Bandi
Department of Metallurgical and Materials Engineering, VNIT Nagpur, Maharashtra 440010, India
Vikram Hastak
Department of Metallurgical and Materials Engineering, VNIT Nagpur, Maharashtra 440010, India
Chokkakula L.P. Pavithra
Department of Materials Science and Metallurgical Engineering, IIT Hyderabad, Telangana 502285, India
Sanjay Kashyap
School of Physics and Materials Science, Thapar Institute of Engineering and Technology, Patiala, Punjab 147004, India
Dhananjay Kumar Singh
Department of Molecular Bioprospection, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh 226002, India; and Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv699780, Israel
Suaib Luqman
Department of Molecular Bioprospection, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh 226002, India
Dilip R. Peshwe
Department of Metallurgical and Materials Engineering, VNIT Nagpur, Maharashtra 440010, India
Ajeet K. Srivastav
Department of Metallurgical and Materials Engineering, VNIT Nagpur, Maharashtra 440010, India
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Superparamagnetic iron oxide nanoparticles are well known for biomedical applications. The particle size, morphology, surface area, and functionalization are the key parameters that affect their bioactivity properties. Inline to this, the superparamagnetic Fe3O4 nanoparticles were prepared via chemical coprecipitation method with an average particle size of 6 ± 3 nm. The particles were surface-functionalized with chitosan and in-house prepared reduced graphene oxide (rGO) to obtain chitosan-coated Fe3O4 nanoparticles (C-Fe3O4) and rGO-Fe3O4 nanocomposites (G-Fe3O4), respectively. Upon functionalization, the physicochemical properties of the materials were characterized thoroughly using X-ray diffraction, transmission electron microscopy, vibrating sample magnetometer, Raman Spectroscopy, and thermal gravimetric analysis. Furthermore, they have subjected to cytotoxicity assay, agar two-fold broth dilution test, and disc diffusion assay experiments for the determination of cytotoxicity and antibacterial activities. The effect of surface functionalization on their bioactivity was investigated thoroughly. The surface functionalization with chitosan and rGO has enhanced the bioactivity of the Fe3O4 nanoparticles.

Copyright © Materials Research Society 2019 

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These authors contributed equally to this work.


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