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Cell labeling efficiency of layer-by-layer self-assembly modified silica nanoparticles

Published online by Cambridge University Press:  31 January 2011

Gang Liu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Jing Tian
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Chen Liu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Hua Ai*
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Zhongwei Gu
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Jilong Gou
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People’s Republic of China
Xianming Mo
Affiliation:
Laboratory of Stem Cell Biology, West China Medical School, Sichuan University, Chengdu 610041, People’s Republic of China
*Corresponding
a) Address all correspondence to this author. e-mail: huaai@scu.edu.cn
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Abstract

In the present study, we compared cytotoxicity and cell uptake of silica nanoparticles with four different surface coatings generated through layer-by-layer self-assembly. Rabbit mesenchymal stem cells (rMSCs) were labeled with silica nanoparticles of different coatings including poly(ethyleneimine) (PEI), poly(allylamine hydrochloride) (PAH), poly(anetholesulfonic acid, sodium salt) (PAS), and dextran sulfate. The MTT [3-(4, 5-dimethylthiazol-2)-2, 5-diphenyl-2H-tetrazolium bromide] test was performed to quantify the cell biocompatibility. The cellular uptake of those silica nanoparticles was determined by flow cytometry and confocal laser scanning microscopy. The results showed that all examined silica nanoparticles were stable in aqueous phase with high monodispersity. Labeled rMSCs are unaffected in their viability, apoptosis, and differentiation capacities. The silica nanoparticle-coated synthetic polycations such as PEI or PAH have higher cell internalization than negatively charged polyelectrolytes. The ability to control cell uptake of different particles may have applications in cell labeling, cell separation, and other biomedical applications.

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Articles
Copyright
Copyright © Materials Research Society 2009

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References

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