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Measurement of small tissue volumes using Holden's apparatus

Published online by Cambridge University Press:  29 June 2007

J. D. T. Mason ,
S. S. Hehar ,
M. Holden  and
N. S. Jones
J. D. T. Mason*
Affiliation:
Department of Otorhinolaryngology, Leicester Royal Infirmary, Leicester.
S. S. Hehar
Affiliation:
Departments of Otorhinolaryngology, University Hospital, Nottingham.
M. Holden
Affiliation:
Department of Medical Physics, University Hospital, Nottingham.
N. S. Jones
Affiliation:
Departments of Otorhinolaryngology, University Hospital, Nottingham.
*
Mr J. D. T. Mason, Department of Otorhinolaryngology, Leicester Royal Infirmary, Leicester LE1 5WW. Fax: 01162 586082
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Abstract

A reproducible method for the estimation of volumes of small tissue samples was needed to allow comparison of the biological activity of tissue explants and to relate symptom improvement to procedures such as adenoidectomy or polypectomy. Holdens apparatus, which utilizes fluid displacement between two chambers, was compared to displacement in a 20 ml syringe. Two observers made four readings each on 20 tissue samples (range 0.37 to 4.70 ml) using both methods for comparison.

A two-way analysis of variance for replicated data gave an F ratio of 0.6885 (df 19; p>0.8) for Holden's apparatus, and an F ratio of 1.4307 (df 19; p>0.1) for the 20 ml syringe. The standard error of the mean for repeated readings on the same sample by an observer was 0.001 ml for Holden's apparatus and 0.014 ml for the syringe method. For accurate estimation of small tissue volumes within 0.01 ml we recommend Holden's apparatus.

Keywords

Tissue, volume estimation
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 12 , December 1995 , pp. 1181 - 1183
Copyright
Copyright © JLO (1984) Limited 1995

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References

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