Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-06-29T09:35:55.703Z Has data issue: false hasContentIssue false
  • English
  • Français

Flow cytometric analysis of DNA content in laryngeal cancer

Published online by Cambridge University Press:  29 June 2007

Raphael Feinmesser ,
Jeremy L. Freeman  and
Arnold Noyek
Raphael Feinmesser*
Affiliation:
Toronto, Canada
Jeremy L. Freeman
Affiliation:
Toronto, Canada
Arnold Noyek
Affiliation:
Toronto, Canada
*
Dr Raphael Feinmesser, MD Department of OtolaryngologyHadasah Medical Centre—Ein KaremPOB 12000Jerusalem 91120Israel
Get access Rights & Permissions [Opens in a new window]

Abstract

DNA content was measured by flow-cytometric analysis in 30 paraffin embedded sections from patients with laryngeal squamous cell carcinoma. The morphological characteristics and N staging of the tumours as registered in their clinical charts were correlated with their DNA content. Eighty per cent of the tumours were found to have a predominantly aneuploid distribution of DNA values. There was no correlation between the N stage of the tumour or degree of cell differentiation and DNA content. A multiploid pattern correlates with non-metastatic laryngeal tumours and we suggest that this pattern may be a good indicator for biological activity.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 104 , Issue 6 , June 1990 , pp. 485 - 487
Copyright
Copyright © JLO (1984) Limited 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Atkin, N. B., Baker, M. C. (1966) Chromosome abnormalities as primary events in human malignant disease: evidence from marker chromosomes. Journal of National Cancer Institute. 36: 539–51.Google ScholarPubMed
Chassevent, A., Daverm, A., Bertrand, G., Coic, , Gesloiss, J., Bidabe, M. C. L., George, P., Larra, F. (1984) Comparative flow DNA analysis of different cell suspensions in breast carcinoma. Cytometry 5: 263–67.CrossRefGoogle ScholarPubMed
Goldsmith, M. N., Cresson, D. H., Postma, D. S., Askin, F. B., Pillsbury, H. D. (1986) Significance of ploidy in laryngeal cancer. American Journal of Surgery, 152: 397–402.CrossRefGoogle ScholarPubMed
Gustafson, H., Tribukait, B., Exposti, P. L. (1982). DNA patterns histological grade and multiplicity related to recurrence rate in superficial bladder tumours. Scandinavian Journal of Urology and Nephrology, 16: 135–39.CrossRefGoogle ScholarPubMed
Hedley, D. W., Friedlander, M. L., Taylor, J. W., Rugg, C. A., Musgrove, E. A. (1985) Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. Journal of Histochemistry and Cytochemistry, 31: 1333–1335.CrossRefGoogle Scholar
Holm, L. E. (1982). Cellular DNA amounts of squamous cell carcinoma of the head and neck region in relation to prognosis. Laryngoscope 92: 1064–69.CrossRefGoogle ScholarPubMed
Johannessen, J. V., Sobrinho-Simoes, M., Lindmo, T., and Tangen, K. O. (1982) The diagnostic value of flow cytometric DNA measurements in selected disorders of the human thyroid. American Journal of Clinical Pathology, 77: 20–25.CrossRefGoogle ScholarPubMed
Kurman, R. J., Carole, G. (1980) Immunoperoxide Technique in Surgical Pathology: Principles and Practice. Manual of Clinical Immunology (eds. Rose, N. R., Friedman, E. H.). 2nd edition, 60–69. American Society of Microbiology, Washington DC.Google Scholar
Miles, C. P. (1967) Chromosome analysis of solid tumours: twentysix epithelial tumours. Cancer, 20: 1274–87.3.0.CO;2-0>CrossRefGoogle Scholar
Nowell, P. C. (1986) Mechanism of tumour progression. Cancer Research, 46: 2203–27.Google Scholar
Sickle-Santanells, B. J., Farrar, W. B., Dobson, J. L. (1986) Analysis of DNA content as a prognostic indicator in squamous cell carcinoma of the tongue. American Journal of Surgery, 152: 393–95.CrossRefGoogle Scholar