Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-22T06:05:29.141Z Has data issue: false hasContentIssue false
  • English
  • Français

Time course of the antibody response in humans compared with rats experimentally infected with hepatic alveolar echinococcosis

Published online by Cambridge University Press:  09 November 2012

K. Yamano ,
M. Miyoshi ,
A. Goto  and
S. Kawase
K. Yamano*
Affiliation:
Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo060-0819, Japan
M. Miyoshi
Affiliation:
Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo060-0819, Japan
A. Goto
Affiliation:
Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo060-0819, Japan
S. Kawase
Affiliation:
Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo060-0819, Japan
*
*Fax: +81-11-736-9476 E-mail: yamano@iph.pref.hokkaido.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

Human alveolar echinococcosis (AE) is caused by the accidental ingestion of Echinococcus multilocularis eggs. Early detection is essential as surgical resection is the only treatment for a complete cure. However, details are unclear about changes in the antibody response during the initial stages of infection, yet such information is useful for early serodiagnosis. Therefore, a long-term investigation was performed into the time course of the antibody response before ‘positive’ detection. Patient sera were used for enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) analysis using crude antigens extracted from E. multilocularis protoscoleces. Rats were experimentally infected with AE and similarly analysed by ELISA and WB. Among the markers for diagnoses, the 18 kDa band (main marker) appeared last in the preoperative stages and disappeared first after operation in a WB test. Although the 18 kDa antigen can be useful as a marker for AE diagnosis, it cannot contribute to the detection of some patients before the 18 kDa band appearance. To avoid misdiagnosis, different diagnostic antigens such as the 26–28 and 7–8 kDa bands should also be considered. These bands tend to appear earlier than the 18 kDa band and thus offer the potential for early detection of AE. We first observed changes in the antibody response in a relatively early stage after infection in human AE cases. Notably, changes in the antibody response of two intermediate species were similar. These findings provide valuable information for the early detection of human AE cases in the future.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 1 , March 2014 , pp. 24 - 31
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Condon, J., Rausch, R.L. & Wilson, J.F. (1988) Application of the avidin–biotin immunohistochemical method for the diagnosis of alveolar hydatid disease from tissue sections. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 731–735.CrossRefGoogle ScholarPubMed
de la Rue, M.L., Yamano, K., Almeida, C.E., Iesbich, M.P., Fernandes, C.D., Goto, A., Kouguchi, H. & Takahashi, K. (2010) Serological reactivity of patients with Echinococcus infections (E. granulosus, E. vogeli, and E. multilocularis) against three antigen B subunits. Parasitology Research 106, 741–745.Google Scholar
Deplazes, P., Grimm, F., Sydler, T., Tanner, I. & Kapel, C.M. (2005) Experimental alveolar echinococcosis in pigs, lesion development and serological follow up. Veterinary Parasitology 130, 213–222.CrossRefGoogle ScholarPubMed
Fujimoto, Y., Ito, A., Ishikawa, Y., Inoue, M., Suzuki, Y., Ohhira, M., Ohtake, T. & Kohgo, Y. (2005) Usefulness of recombinant Em18-ELISA to evaluate efficacy of treatment in patients with alveolar echinococcosis. Journal of Gastroenterology 40, 426–431.Google Scholar
Furuya, K., Sato, H. & Kumagai, M. (1987) Application of Western blotting method for immunoserologic diagnosis of hydatidosis. Journal of Clinical and Experimental Medicine (IGAKU NO AYUMI) 141, 41–42 (in Japanese).Google Scholar
Furuya, K., Sato, N., Kawanaka, M., Takahashi, K., Sawada, Y., Honma, H. & Tamura, M. (2001) Immunohistochemical examinations of echinococcal liver lesions from patients with alveolar hydatid disease in Hokkaido. Report of Hokkaido Institute of Public Health 51, 1–6(in Japanese with English abstract).Google Scholar
Ito, A., Nakao, M., Kutsumi, H., Lightowlers, M.W., Itoh, M. & Sato, S. (1993) Serodiagnosis of alveolar hydatid disease by Western blotting. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 170–172.Google Scholar
Ito, A., Schantz, P.M. & Wilson, J.F. (1995) EM18, a new serodiagnostic marker for differentiation of active and inactive cases of alveolar hydatid disease. American Journal of Tropical Medicine and Hygiene 52, 41–44.Google Scholar
Ito, A., Sako, Y., Yamasaki, H., Mamuti, W., Nakaya, K., Nakao, M. & Ishikawa, Y. (2003) Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis. Acta Tropica 85, 173–182.Google Scholar
Kimura, H., Furuya, K., Kawase, S., Sato, C., Yamano, K., Takahashi, K., Uraguchi, K., Ito, T., Yagi, K. & Sato, N. (1999) Recent epidemiologic trends in alveolar echinococcosis prevalence in humans and animals in Hokkaido. Journal of the Japanese Association for Infectious Diseases 52, 117–120.Google Scholar
Kishimoto, M., Yamada, K., Yamano, K., Kobayashi, N., Fujimoto, S., Shimizu, J., Lee, K.J., Iwasaki, T. & Miyake, Y. (2009) Significance of imaging features of alveolar echinococcosis in studies on nonhuman primates. American Journal of Tropical Medicine and Hygiene 81, 540–544.CrossRefGoogle ScholarPubMed
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.Google Scholar
Leggatt, G.R., Yang, W. & McManus, D.P. (1992) Serological evaluation of the 12 kDa subunit of antigen B in Echinococcus granulosus cyst fluid by immunoblot analysis. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 1–4.Google Scholar
Liance, M., Janin, V., Bresson-Hadni, S., Vuitton, D.A., Houin, R. & Piarroux, R. (2000) Immunodiagnosis of Echinococcus infections: Confirmatory testing and species differentiation by new commercial Western blot. Journal of Clinical Microbiology 38, 3718–3721.Google Scholar
Lightowlers, M.W., Mitchell, G.F. & Rickard, M.D. (1993) Immunology and molecular biology of parasitic infections. pp. 438–472in Warren, K.S. & Agabian, N. (Eds) Cestodes. Oxford, Blackwell Scientific.Google Scholar
Sato, C., Kawase, S., Yano, S., Nagano, H., Fujimoto, S., Kobayashi, N., Miyahara, K., Yamada, K., Sato, M. & Kobayashi, Y. (2005) Outbreak of larval Echinococcus multilocularis infection in Japanese monkey (Macaca fuscata) in a zoo, Hokkaido: western blotting patterns in the infected monkeys. Journal of Veterinary Medical Science 67, 133–135.CrossRefGoogle Scholar
Sato, H., Mitamura, H., Arai, J. & Kumagai, M. (1983) Serological diagnosis of human hydatid diseases by enzyme-linked immunosorbent assay (Part 1): Enzyme-linked immunosorbent assay by multilocular Echinococcus antigen. Report of Hokkaido Institute of Public Health 33, 8–15(in Japanese with English summary).Google Scholar
Tappe, D., Grüner, B., Kern, P. & Frosch, M. (2008) Evaluation of a commercial Echinococcus western blot assay for serological follow-up of patients with alveolar echinococcosis. Clinical and Vaccine Immunology 15, 1633–1637.Google Scholar
Tappe, D., Frosch, M., Sako, Y., Itoh, S., Grüner, B., Reuter, S., Nakao, M., Ito, A. & Kern, P. (2009) Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages. American Journal of Tropical Medicine and Hygiene 80, 792–797.Google Scholar
Torgerson, P.R. & Budke, C. (2003) Echinococcosis – an international public health challenge. Research in Veterinary Science 74, 191–202.Google Scholar
Torgerson, P.R., Schweiger, A., Deplazes, P., Pohar, M., Reichen, J., Ammann, R.W., Tarr, P.E., Halkik, N. & Müllhaupt, B. (2008) Alveolar echinococcosis: from a deadly disease to a well-controlled infection. Relative survival and economic analysis in Switzerland over the last 35 years. Journal of Hepatology 49, 72–77.Google Scholar
Wen, H., New, R.R.C. & Craig, P.S. (1993) Diagnosis and treatment of human hydatidosis. British Journal of Clinical Pharmacology 35, 565–574.Google Scholar
Yamano, K., Yagi, K., Furuya, K., Sawada, Y., Honma, H. & Sato, N. (2005) Active alveolar hydatidosis with sero-negativity for antibody to the 18 kDa antigen. Journal of the Japanese Association for Infectious Diseases 58, 122–124.Google Scholar
Yamano, K., Hada, N., Yamamura, T., Takeda, T., Honma, H. & Sawada, Y. (2006) Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis. Journal of Helminthology 80, 387–391.Google Scholar
Yamano, K., Goto, A., Nakamura-Uchiyama, F., Nawa, Y., Hada, N. & Takeda, T. (2009a) Galβ1-6Gal, antigenic epitope which accounts for serological cross-reaction in diagnosis of Echinococcus multilocularis infection. Parasite Immunology 31, 481–487.Google Scholar
Yamano, K., Goto, A., Miyoshi, M., Furuya, K., Sawada, Y. & Sato, N. (2009b) Diagnosis of alveolar echinococcosis using immunoblotting with plural low molecular weight antigens. Journal of Helminthology 83, 57–61.Google Scholar
Yamano, K., Kanetoshi, A., Goto, A., Kishimoto, M., Kobayashi, N., Fujimoto, S. & Yamada, K. (2009c) Japanese monkey (Macaca fuscata) with alveolar echinococcosis after treatment with albendazole for 10 years: serodiagnosis and determination of albendazole metabolites. Parasitology Research 106, 69–74.CrossRefGoogle ScholarPubMed