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Phylogenetic position of Sphincterodiplostomum Dubois, 1936 (Digenea: Diplostomoidea) with description of a second species from Pantanal, Brazil

Published online by Cambridge University Press:  11 February 2021

T.J. Achatz Open the ORCID record for T.J. Achatz [Opens in a new window] ,
J.A. Bell Open the ORCID record for J.A. Bell [Opens in a new window] ,
F.T.V. Melo Open the ORCID record for F.T.V. Melo [Opens in a new window] ,
A. Fecchio Open the ORCID record for A. Fecchio [Opens in a new window]  and
V.V. Tkach Open the ORCID record for V.V. Tkach [Opens in a new window]
T.J. Achatz
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
J.A. Bell
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
F.T.V. Melo
Affiliation:
Laboratory of Cell Biology and Helminthology ‘Prof. Dr Reinalda Marisa Lanfredi’, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil
A. Fecchio
Affiliation:
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil
V.V. Tkach*
Affiliation:
Department of Biology, University of North Dakota, Starcher Hall, 10 Cornell Street Stop 9019, Grand Forks, ND58202, USA
*
Author for correspondence: V.V. Tkach, E-mail: vasyl.tkach@und.edu
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Abstract

Sphincterodiplostomum is a monotypic genus of diplostomid digeneans that parasitize fish-eating birds in the neotropics. The type species Sphincterodiplostomum musculosum has a unique, dorsal, tubular invagination in the opisthosoma with a muscular sphincter. Whereas larvae of S. musculosum are relatively commonly reported in Neotropical fish helminth surveys, adult specimens from birds are rarely collected. Prior to our study, no DNA sequence data for S. musculosum were available. Our molecular and morphological study of mature and immature adult Sphincterodiplostomum specimens from three species of birds and one species of crocodilian revealed the presence of at least two species of Sphincterodiplostomum in the neotropics. We provide the first molecular phylogeny of the Diplostomoidea that includes Sphincterodiplostomum. In addition, this is the first record of S. musculosum from caimans, along with the first record of fully mature adult S. musculosum from green kingfisher Chloroceryle americana. The new species of Sphincterodiplostomum (Sphincterodiplostomum joaopinhoi n. sp.) can be morphologically distinguished from S. musculosum based on the anterior extent of vitelline follicles, narrower prosoma, substantially smaller holdfast organ and structure of tegumental spines. Our data revealed 0.7% interspecific divergence in 28S and 10.6–11.7% divergence in cox1 sequences between the two Sphincterodiplostomum species.

Keywords

DiplostomidaeSphincterodiplostomum musculosumSphincterodiplostomum joaopinhoi n. spmolecular phylogenyPantanal
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e6
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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