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Methods for quantifying eggs and oviposition rate of Dawestrema cycloancistrium (Monogenea: Dactylogyridae), monogenean parasite of Arapaima gigas (Teleostei: Osteoglossidae)

Published online by Cambridge University Press:  26 October 2018

P.O. Maciel Open the ORCID record for P.O. Maciel [Opens in a new window]  and
R.R. Alves Open the ORCID record for R.R. Alves [Opens in a new window]
P.O. Maciel*
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pesca e Aquicultura, Postal Box 90, 77008-900, Palmas, TO, Brazil
R.R. Alves
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Pesca e Aquicultura, Postal Box 90, 77008-900, Palmas, TO, Brazil
*
Author for correspondence: P.O. Maciel, E-mail: patricia.maciel@embrapa.br
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Abstract

Dawestrema cycloancistrium is the main ectoparasite causing mortality in fingerlings of pirarucu (Arapaima gigas) in Amazonian aquaculture. Very little is known about the D. cycloancistrium reproductive index and appropriate methods of collecting eggs for investigation. This study aimed to determine the oviposition rate of D. cycloancistrium. To achieve this aim, two egg quantification methods were tested: the estimative method (ME) and the total counting method (MT). Compared with the MT, the ME overestimated the number of eggs counted, which were 2943.5 ± 2840.6 and 1041.5 ± 533 eggs, and the oviposition rate, which was 80.1 ± 58.7 and 31.4 ± 16.4 eggs/parasite/day, for ME and MT, respectively. These results show that for studies quantifying D. cycloancistrium eggs, the total eggs in the sample must be counted, as the estimates made using subsamples are not representative. Using the MT, the oviposition rate for D. cycloancistrium was determined to be 31.4 ± 16.4 eggs per adult parasite per day. The present study demonstrates the egg production capacity of a monogenean species parasite of A. gigas, providing basic biological data for D. cycloancistrium.

Keywords

aquacultureDactylogyridaeegg collectionfish diseasegill parasiteMonogeneapaichepirarucu
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e4
Copyright
Copyright © Cambridge University Press 2018 

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