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Intra-species variability in ITS-1 sequences of Haemonchus contortus isolated from goats in West Bengal, India

Published online by Cambridge University Press:  31 August 2010

S. Bandyopadhyay ,
A.K. Bera ,
S. Sikdar ,
S. De ,
S. Das ,
T. Rana ,
D. Pan ,
S. Bandyopadhyay  and
D. Bhattacharya
S. Bandyopadhyay*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
A.K. Bera
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Sikdar
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. De
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Das
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
T. Rana
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
D. Pan
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
S. Bandyopadhyay*
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
D. Bhattacharya
Affiliation:
Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata37, West Bengal, India
*
*E-mail: subuicar@rediffmail.com
*E-mail: subuicar@rediffmail.com
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Abstract

This study evaluated the existence of different genotypes of Haemonchus contortus prevailing among goats in West Bengal, India. These parasites were isolated from the abomasum of goat intestine and the molecular characterization was performed by comparing variation of nucleotide sequences of the internal transcribed spacer 1 (ITS-1) gene region. Single-strand conformation polymorphism (SSCP) analysis of ITS-1 amplified product showed the presence of three distinct conformations both in male and female parasites. The sequence analysis of conformations showed two single nucleotide polymorphisms (SNP) in male parasites at nucleotide positions 106 and 107 and one SNP was detected in female parasites at nucleotide position 157. These nucleotide variations in different isolates did not alter the interior loop structure of the predicted secondary RNA, therefore we believe these variations may not be responsible for any evolutionary changes among conformations.

Type
Regular research papers
Information
Journal of Helminthology , Volume 85 , Issue 2 , June 2011 , pp. 204 - 209
Copyright
Copyright © Cambridge University Press 2010

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References

Bhargavi, R., Vishwakarma, S. & Murty, U.S. (2005) A secondary structural common core in the ribosomal ITS2 (internal transcribed spacer) of Culex species from diverse geographical locations. Bioinformation 1, 52–55.CrossRefGoogle ScholarPubMed
Blok, V.C., Malloch, G., Harrower, B. & Phillips, M.S. (1998) Intraspecies variation in ribosomal DNA in populations of the potato cyst nematode Globodera pallida. Journal of Nematology 30, 262–274.Google Scholar
Campbell, C.S., Wright, W.A., Cox, M., Vining, T.F., Major, C.S. & Arsenault, M.P. (2005) Nuclear ribosomal DNA internal transcribed spacer 1 (ITS-1) in Picea (Pinaceae): sequence divergence and structure. Molecular Phylogenetic Evolution 35, 165–185.CrossRefGoogle Scholar
Dover, G.A. (1982) Molecular drive: a cohesive mode of species evolution. Nature 299, 111–117.CrossRefGoogle ScholarPubMed
Fang, X., Luo, Z., Yuan, B. & Wang, J. (2007) Improving the prediction of RNA secondary structure by detecting and assessing conserved stems. Bioinformation 30, 222–229.CrossRefGoogle Scholar
Fritz, G.N., Conn, J., Cockburn, A. & Seawright, J. (1994) Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuenztovari (Diptera: Culicidae). Molecular Biology and Evolution 11, 406–416.Google ScholarPubMed
Gandolfi, A., Bonilauri, P., Rossi, V. & Menozzi, P. (2001) Intraindividual and intraspecies variability of ITS-1 sequences in the ancient asexual Darwinula stevensoni (Crustacea: Ostracoda). Heredity 87, 449–455.CrossRefGoogle Scholar
Gruber, A.R., Lorenz, R., Bernhart, S.H., Neuböck, R. & Hofacker, I.L. (2008) The Vienna RNA Websuite. Nucleic Acids Research 36 (Web Server issue), W70–W74.CrossRefGoogle ScholarPubMed
Harris, D.J. & Crandall, K.A. (2000) Intragenomic variation within ITS-1 and ITS-2 of freshwater crayfishes (Decapoda: Cambaridae): implications for hylogenetic and microsatellite studies. Molecular Biology Evolution 17, 284–291.CrossRefGoogle Scholar
Kia, E.B., Rahijmi, H.R., Mirhendi, S.H., Talebi, A., Fasihi Harandi, M., Jalalizand, N. & Rokni, M.B. (2007) A new primer pair in ITS-1 region for molecular studies on Echinococcus granulosus. Iranian Journal of Public Health 36, 45–49.Google Scholar
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111–120.CrossRefGoogle ScholarPubMed
Lalev, A.I. & Nazar, R.N. (1999) Structural equivalence in the transcribed spacers of pre-rRNA transcripts in Schizosaccharomyces pombe. Nucleic Acids Research 27, 3071–3078.CrossRefGoogle ScholarPubMed
Lau, C.F. & Sheu, S.S. (1992) Rapid and direct recovery of DNA fragments from agarose gels: an extremely simple method. Methods in Molecular Cell Biology 3, 190–192.Google Scholar
Miller, J.E. & Horohov, D.W. (2006) Immunological aspects of nematode parasite control in sheep. Journal of Animal Science 84, E124–E132.CrossRefGoogle ScholarPubMed
Navajas, M., Lagnel, J., Fauvel, G. & De Moraes, G. (1999) Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites. Experimental Applied Acarology 23, 851–859.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Fantaccione, S. & Woodrow, P. (2008) Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS-1 (internal transcribed spacer 1) secondary structure prediction. Bioinformation 2, 311–315.Google Scholar
Queipo-ortuno, M.I., Colmenero, J.D.D., Macias, M., Bravo, M.J. & Morata, P. (2008) Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as a real-time PCR for serum sample from patients with brucellosis. Clinical Vaccine Immunology 15, 293–296.CrossRefGoogle ScholarPubMed
Rahman, W.A., Hamid, S.A. & Othman, A.S. (2007) Genetic characterization of Haemonchus contortus in goats (Capra hircus) and sheep (Ovis aries) in Penang, Malaysia. Journal of Bioscience 18, 45–56.Google Scholar
Smit, S., Widmann, J. & Knight, R. (2007) Evolutionary rates vary among rRNA structural elements. Nucleic Acids Research 35, 3339–3354.CrossRefGoogle ScholarPubMed
Szalanski, A.L., Roehrdanz, R.L., Taylor, D.B. & Chandler, L. (1999) Genetic variation in geographical populations of western and Mexican corn rootworm. Insect Molecular Biology 8, 519–525.CrossRefGoogle ScholarPubMed
Van Herwerden, L., Blair, D. & Agatsuma, T. (1999) Intra and interindividual variation in ITS-1 of Paragonimus westermani (Trematoda: Digenea) and related species: implications for phylogenetic studies. Molecular Phylogeny and Evoution 12, 66–78.Google Scholar
Xie, T., Ho, S.L. & Ma, O.C. (1997) High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining. Molecular Pathology 50, 276–278.CrossRefGoogle ScholarPubMed