Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-05-03T20:23:31.824Z Has data issue: false hasContentIssue false
  • English
  • Français

Delayed macrofilaricidal activity of diethylcarbamazine against Brugia pahangi in Mongolian jirds

Published online by Cambridge University Press:  12 April 2024

Y. Fujimaki ,
P. Sithithaworn ,
Y. Mitsui  and
Y. Aoki
Y. Fujimaki*
Affiliation:
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, 852-8523, Japan
P. Sithithaworn
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand
Y. Mitsui
Affiliation:
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, 852-8523, Japan
Y. Aoki
Affiliation:
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, 852-8523, Japan
*
*Fax: + 81 95 849 7805 E-mail: fujimaki@net.nagasaki-u.ac.
Get access Rights & Permissions [Opens in a new window]

Abstract

The macrofilaricidal activity of diethylcarbamazine (DEC) was confirmed in jirds infected with Brugia pahangi. Seventy jirds were inoculated subcutaneously with 100 infective larvae. At 20 weeks post-infection, the microfilaraemic jirds were divided into two groups, untreated and treated. For the treated group, 200 mg kg−1 of DEC was injected intraperitoneally for 5 consecutive days. One, 4, 8, 12, 16 and 27 weeks after the final treatment, 4–7 jirds in each group were sacrificed to measure adult worm burdens. The number of adult worms recovered from treated jirds was comparable to controls at earlier necropsy (1 and 4 weeks post-treatment). However, at late necropsy (8 weeks and later) the recovery rate of adult worms in treated jirds was significantly lower than that in untreated controls, indicating an adultcidal effect of DEC. The present study demonstrates that DEC requires 8 weeks to kill B. pahangi adult worms in jirds and that the Mongolian jird is a useful model for screening antifilarial activity.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 4 , December 2004 , pp. 293 - 295
Copyright
Copyright © Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ash, L.R. & Riley, J.M. (1970) Development of Brugia pahangi in the jird, Meriones unguiculatus, with notes on infections in other rodents. Journal of Parasitology 56, 962–968.CrossRefGoogle ScholarPubMed
Denham, D.A., Suswillo, R.R., Rogers, R., McGreevy, P.N.B. & Andrews, B.J. (1976) Studies on Brugia pahangi 13. The anthelmintic effect of compounds F151 (Friedheim), HOE 33258 (Hochest) and their reaction product. Journal of Helminthology 50, 243–250.CrossRefGoogle Scholar
Denham, D.A., Suswillo, R.R., Rogers, R. & McGreevy, P.N.B. (1978) Studies on Brugia pahangi. 17. The anthelmintic effects of diethylcarbamazine. Journal of Parasitology 64, 463–468.CrossRefGoogle ScholarPubMed
Denham, D.A., Midwinter, I.T. & Friedheim, E.A. (1990) The anthelmintic activity of a novel organic arsenical, R7/45, upon Brugia pahangi in Meriones unguiculatus . Journal of Helminthology 64, 100–104.CrossRefGoogle ScholarPubMed
Duke, B.O. (1991) Suramin and the time it takes to kill Onchocerca volvulus . Tropical Medicine and Parasitology 42, 346–350.Google ScholarPubMed
Hewitt, R.I., Kushber, S., Stewart, H.W., Whote, E., Wallace, W.S. & Subbarow, Y. (1947) Experimental chemotherapy of filariasis III. Effect of 1-diethylcarbamol-4-methylpiperazine hydrochloride against naturally acquired filarial infections in cotton rats and dogs. Journal of Laboratory and Clinical Medicine 23, 1314–1329.Google Scholar
Kimura, E., Aoki, Y., Nakajima, Y & Niwa, M. (1984) Plasma level of diethylcarbamazine in jirds and hamsters. Southeast Asian Journal of Tropical Medicine and Public Health 15, 74–79.Google ScholarPubMed
Mitsui, Y., Takamura, N., Fujimaki, Y., Yamaguchi, T., Kitagawa, T. & Aoki, Y. (1996) Development of a competitive enzyme-linked immunosorbent assay for diethylcarbamazine. Tropical Medicine and International Health 1, 528–534.CrossRefGoogle ScholarPubMed
Shibuya, T., Oikawa, Y., Sato, M. & Tanaka, H. (1979) Mebendazole–a slow acting macro- and microfilaricide against Litomosoides carinii in cotton rats. Japanese Journal of Experimental Medicine 49, 351–354.Google Scholar
Shigeno, S., Kimura, E., Sakamoto, M. & Aoki, Y. (1983) Effect of diethylcarbamazine on third and fourth stage larvae of Brugia pahangi in Mongolian jird. Japanese Journal of Parasitology 32, 465–473.Google Scholar
Surin, J. & Denham, D.A. (1990) Comparative susceptibility to anthelmintics of Brugia pahangi in jirds infected by different methods. Journal of Helminthology 64, 232–238.CrossRefGoogle ScholarPubMed
Suswillo, R.R. & Denham, D.A. (1977) A new system of testing for filaricidal activity using transplanted adult Brugia in the jird. Journal of Helminthology 63, 591–592.Google ScholarPubMed
Tropical Diseases Research (1997) Special programme for research and training in tropical diseases. Workplan of the steering committee on macrofilaricidal drugs for onchocerciasis and lymphatic filariasis (macrofil).Google Scholar
Tyagi, K., Murthy, P.K., Chowdhury, T.K.R., Chatterjee, R.K. & Seu, A.P. (1986) Chemotherapeutic response of Brugia malayi to antifilarials in Mastomys natalensis . Indian Journal Parasitology 10, 195–207.Google Scholar
Yamashita, S., Shigeno, S., Kimura, E. & Aoki, Y. (1983) The effect of diethylcarbamazine on microfilariae of Brugia pahangi in Mongolian jirds (Meriones unguiculatus) . Tropical Medicine 25, 113–117.Google Scholar
Zahner, H. & Schares, G. (1993) Experimental chemotherapy of filariasis: comparative evaluation of the efficacy of filaricidal compounds in Mastomys coucha infected with Litomosoides carinii, Acanthocheilonema viteae, Brugia malayi and B. pahangi . Acta Tropica 52, 221–226.CrossRefGoogle ScholarPubMed