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Comparative study on polypeptide patterns of larvae of Trichinella isolates by two-dimensional electrophoresis

Published online by Cambridge University Press:  05 June 2009

T. Garate  and
L. Rivas
T. Garate
Affiliation:
Departmento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040-Madrid, Spain
L. Rivas
Affiliation:
Unidad de Biomembranas, Instituto de Immunología y Biología Microbiana, Velázquez, 144, 28006–Madrid, Spain
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Abstract

The two-dimensional patterns (isoelectrofocusing-IEF/polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate-SDS) of S3 fractions of muscle larvae of four Trichinella isolates were compared. The comparative study concerned six groups of polypeptides. It was observed that the Garkavi isolate of Trichinella pseudospiralis was clearly different from the other isolates, and it showed the simplest IEF/SDS polypeptide pattern. The C-76 isolate of T. nelsoni had only four of the six groups, distinguishing it from the GM-1 isolate of T. spiralis and the Boev isolate of T. nativa that showed all the indicated groups.

Keywords

Trichinella spiralisTrichinella nelsoniTrichinella nativaTrichinella pseudospiralispolypeptideselectrophoresis
Type
Research Note
Information
Journal of Helminthology , Volume 61 , Issue 3 , September 1987 , pp. 225 - 228
Copyright
Copyright © Cambridge University Press 1987

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References

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