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The anthelmintic efficacy of natural plant cysteine proteinases against the rat tapeworm Hymenolepis diminutain vivo

Published online by Cambridge University Press:  12 March 2015

F. Mansur ,
W. Luoga ,
D.J. Buttle ,
I.R. Duce ,
A. Lowe  and
J.M. Behnke
F. Mansur
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia (USIM), Kuala Lumpur, Malaysia
W. Luoga
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK Department of Life Sciences, Mkwawa University College of Education, Iringa, Tanzania
D.J. Buttle
Affiliation:
Department of Infection and Immunity, University of Sheffield Medical School, Beech Hill Road, Sheffield, UK, S10 2RX
I.R. Duce
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
A. Lowe
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
J.M. Behnke*
Affiliation:
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
*
*Fax: 44 115 951 3251 E-mail: jerzy.behnke@nottingham.ac.uk
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Abstract

Hymenolepis diminuta is a natural parasite of the common brown rat Rattus norvegicus, and provides a convenient model system for the assessment of the anthelmintic activity of novel drugs against cestodes. The experiments described in this paper indicate that treatment of rats infected with H. diminuta with a supernatant extract of papaya latex, containing a mixture of four cysteine proteinases, was moderately efficacious, resulting in a significant, but relatively small, reduction in worm burden and biomass. However, faecal egg output was not affected by treatment. In our experiments these effects were only partially dose-dependent, although specific inhibition by E-64 confirmed the role of cysteine proteinases as the active principles in papaya latex affecting worm growth but not statistically reducing worm burden. Data collected for a further 7 days after treatment indicated that the effects of papaya latex supernatant on worm loss and on worm growth were not enhanced. Our findings provide a starting point for further refinement in formulation and delivery, or assessment of alternative natural plant-derived cysteine proteinases in efforts to develop these naturally occurring enzymes into broad-spectrum anthelmintics, with efficacy against cestodes as well as nematodes.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 3 , May 2016 , pp. 284 - 293
Copyright
Copyright © Cambridge University Press 2015 

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