Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-17T09:07:46.103Z Has data issue: false hasContentIssue false
  • English
  • Français

Angiostrongylus costaricensis egg antigen for the immunodiagnosis of abdominal angiostrongyliasis

Published online by Cambridge University Press:  01 September 2008

P. Mesén-Ramírez ,
E. Abrahams-Sandí ,
K. Fernández-Quesada  and
P. Morera
P. Mesén-Ramírez
Affiliation:
Department of Parasitology, University of Costa Rica, San Pedro/Mts. Oca, Costa Rica
E. Abrahams-Sandí*
Affiliation:
Department of Parasitology, University of Costa Rica, San Pedro/Mts. Oca, Costa Rica Pathology Service San Juan de Dios Hospital, San José, Costa Rica
K. Fernández-Quesada
Affiliation:
Microbiology Department, Boston Scientific Costa Rica, 302 Global Parkway, Heredia, Costa Rica
P. Morera
Affiliation:
Pathology Service San Juan de Dios Hospital, San José, Costa Rica
*
*Fax: +506-225-2374elizabeth.abrahams@ucr.ac.cr
Get access Rights & Permissions [Opens in a new window]

Abstract

Angiostrongylus costaricensis is the aetiological agent of human abdominal angiostrongyliasis, a parasitic disease reported from the United States to Argentina, with a widespread occurrence of the nematode throughout Central and South America. This study assesses the performance of A. costaricensis eggs as antigen in an enzyme-linked immunosorbent assay (ELISA), for the determination of parasite-specific IgG1 antibodies. The specificity and the sensitivity of the method were 87% and 90.5%, respectively. Through this test it was possible to demonstrate a sharp and early decline in IgG1 antibody in serum samples taken from patients with histopathological diagnosis of abdominal angiostrongyliasis at different time points after surgical treatment. The present work demonstrated the usefulness of the egg antigen in the development of a specific diagnostic test for abdominal angiostrongylosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 82 , Issue 3 , September 2008 , pp. 251 - 254
Copyright
Copyright © Cambridge University Press 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abrahams, S.E., Geiger, S.M., Fernández, Q.K. & Schulz-Key, H. (2005) Specific antibody production against different life cycle stages during an experimental A. costaricensis infection in mice. Revista Biomédica 16, 239–246.Google Scholar
Abrahams-Sandí, E., Hoffmann, W.H., Graeff-Teixeira, C., Schulz-Key, H. & Geiger, S.M. (2004) Long-term observations on mouse strains experimentally infected with Angiostrongylus costaricensis. Parasitology Research 93, 230–234.CrossRefGoogle ScholarPubMed
Alarcón de Noya, B., Colmenares, C., Lanz, H., Caracciolo, M.A., Losada, S. & Noya, O. (2000) Schistosoma mansoni: immunodiagnosis is improved by sodium metaperiodate which reduces cross-reactivity due glycosylated epitopes of soluble egg antigen. Experimental Parasitology 95, 106–112.CrossRefGoogle ScholarPubMed
Bender, A.L., Maurer, R.L., Fernandes da Silva, M.C., Ben, R., Barros, T.P., Aramburu da Silva, A.C. & Graeff-Teixeira, C. (2003) Ovos e órgãos reprodutores de fêmeas de Angiostrongylus costaricensis são reconhecidos mais intensamente por soros humanos de fase aguda na angiostrongilíase abdominal. Revista da Sociedade Brasileira de Medicina Tropical 36, 449–454.CrossRefGoogle ScholarPubMed
Colmenares, C., Fermin, Z., Lozada, S., Spencer, L., Masrova, G., Noya, O. & Alarcon de Noya, B. (1993) Reactividad cruzada en el immunodiagnóstico de la esquistosomiasis mansoni. Acta Cientifica Venezolana 44, 211.Google Scholar
Cypess, R.H., Karol, M.H., Zidian, J.L., Glickman, L.T. & Gidin, D. (1977) Larva-specific antibodies in patients with visceral larva migrans. Journal of Infectious Diseases 135, 633–640.Google Scholar
Geiger, S.M., Graeff-Teixeira, C., Soboslay, P.T. & Schulz-Key, H. (1999) Experimental Angiostrongylus costaricensis infection in mice: immunoglobulin isotype responses and parasite-specific antigen recognition after primary low dose infection. Parasitology Research 85, 200–205.Google Scholar
Geiger, S.M., Abrahams-Sandí, E., Soboslay, P.T., Hoffmann, W.H., Pfaff, A.W., Graeff-Teixeira, C. & Schulz-Key, H. (2001) Cellular immune responses and cytokine production in BALB/c and C57BL/6 mice during the acute phase of Angiostrongylus costaricensis. Acta Tropica 80, 59–68.CrossRefGoogle ScholarPubMed
Glickman, T.L., Schantz, P., Dombroske, R. & Cypess, R. (1978) Evaluation of serodiagnostic tests for visceral larva migrans. American Journal of Tropical Medicine and Hygiene 27, 492–498.Google Scholar
Graeff-Teixeira, C., Agostini, A.A., Camillo-Coura, L. & Ferreira-Da-Cruz, M.F. (1997) Seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in southern Brazil. Tropical Medicine International Health 2, 254–260.Google Scholar
Morera, P. (1985) Abdominal angiostrongyliasis: a problem of public health. ParasitologyToday 1, 173–175.Google ScholarPubMed
Morera, P. (1987) Angiostrongiliasis abdominal, Un problema de salud pública? Revista Asociación Guatemalteca de Parasitología y Medicina Tropical 2, 9–11.Google Scholar
Morera, P. (1995) Abdominal angiostrongyliasis. pp. 225–230 in Forthing, M.G.J., Keusch, G.T. & Wabelin, D. (Eds) Enteric infection: intestinal helminths. London, Chapman, Hall.Google Scholar
Morera, P. & Amador, J.A. (1998) Prevalencia de la angiostrongilosis abdominal y la distribución estacional de la precipitación. Revista Costarricense de Salud Pública 7, 1–14.Google Scholar
Pollard, Z.F., Jarret, W.H., Hagler, W.S., Allain, D.S. & Schantz, P.M. (1979) ELISA for diagnosis of ocular toxocariasis. Ophthalmology 86, 743–749.CrossRefGoogle ScholarPubMed