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Acute-phase responses in cattle infected with hydatid cysts and microbial agents

Published online by Cambridge University Press:  13 May 2014

A. Sevimli ,
F.K. Sevimli ,
E. Şeker ,
A. Ulucan  and
H.H. Demirel
A. Sevimli*
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
F.K. Sevimli
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
E. Şeker
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Afyon Kocatepe Üniversity, Afyonkarahisar, Turkey
A. Ulucan
Affiliation:
Department of Pathology Laboratory Techniques, Vocational School of Health Services, Bingol University, Bingol, Turkey
H.H. Demirel
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
*
* Fax: +90 272 2281349 E-mail: alpers@aku.edu.tr
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Abstract

The aim of this study was to investigate the effect of hydatid cysts and microbial agents on the acute-phase response in cattle. Twenty-seven cattle with hydatid cysts and eight apparently healthy cattle comprised the study and control groups, respectively. Parasitological, microbiological, histopathological and immunohistochemical examinations of the liver and lungs were undertaken, and 49 of these organs were infected with cysts. In 14 of 31 (45.1%) livers and 10 of 18 (55.5%) lungs microbial growth was observed. The most frequent species occurring in the liver were Staphylococcus aureus, Escherichia coli, Corynebacterium spp. and Campylobacter spp., whereas in the lungs the most common species was Candida spp., followed by Streptococcus spp., Mannheimia haemolytica, Corynebacterium spp., Micrococcus spp. and S. aureus. The concentration of serum interleukin (IL-6) in infected cattle, 455.35 ± 39.68 pg/ml, was significantly higher than that of 83.02 ± 17.87 pg/ml in the control group (P< 0.001). The serum amyloid A (SAA) level of infected cattle was 7.51 ± 0.41 μg/ml, and 4.84 ± 0.51 μg/ml in the control group (P< 0.001). The serum haptoglobin level of infected cattle was found to be 2.08 ± 0.65 ng/ml, while that of the control group was determined as 3.87 ± 0.91 ng/ml (P>0.05). The highest concentrations of IL-6 were detected in serum of the cattle where microbial growth had been detected, followed by cattle infected with bacteria + Trichostrongylus sp. (P< 0.001). Consequently, SAA showed an important increase in the group infected with hydatid cysts, whereas haptoglobin level decreased. It was noticed that IL-6, like SAA, had a significant role in hydatid cyst infection. Therefore IL-6 and SAA appear to be major markers in the detection of infection of cattle with hydatid cysts.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 4 , July 2015 , pp. 471 - 479
Copyright
Copyright © Cambridge University Press 2014 

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