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Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)

Published online by Cambridge University Press:  14 April 2015

V. Vidal
Affiliation:
Departamento de Sanidad Animal (Parasitología), Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, 30100, Espinardo (Murcia), Spain Departamento de Ecología Evolutiva, Museo Nacional de Ciencias Naturales, MNCN-CSIC, José Gutiérrez Abascal, 2, 28006, Madrid, Spain Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia, Spain
J. Ortiz
Affiliation:
Departamento de Sanidad Animal (Parasitología), Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, 30100, Espinardo (Murcia), Spain Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia, Spain
J.I. Diaz
Affiliation:
Centro de Estudios Parasitológicos y de Vectores (CCT CONICET La Plata-CONICET-UNLP), Calle 120 s/n, entre 61 y 62, 1900 La Plata, Argentina
B. Zafrilla
Affiliation:
Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante), Spain
M.J. Bonete
Affiliation:
Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante), Spain
M.R. Ruiz De Ybañez
Affiliation:
Departamento de Sanidad Animal (Parasitología), Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, 30100, Espinardo (Murcia), Spain Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia, Spain
M.J. Palacios
Affiliation:
Departamento de Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas, EEZA-CSIC, Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain
J. Benzal
Affiliation:
Departamento de Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas, EEZA-CSIC, Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain
F. Valera
Affiliation:
Departamento de Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas, EEZA-CSIC, Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain
C. De La Cruz
Affiliation:
Departamento de Biología Animal, Facultad de Ciencias, Universidad de Extremadura, 06006, Badajoz, Spain
M. Motas
Affiliation:
Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia, Spain Departamento de Ciencias Sociosanitarias (Toxicología), Facultad de Veterinaria, Universidad de Murcia, Campus de Excelencia Internacional Regional ‘Campus Mare Nostrum’, 30100, Espinardo (Murcia), Spain
V. Bautista
Affiliation:
Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Ctra. San Vicente del Raspeig s/n, 03690, San Vicente del Raspeig (Alicante), Spain
A. Machordom
Affiliation:
Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, MNCN-CSIC, José Gutiérrez Abascal, 2, 28006, Madrid, Spain
A. Barbosa
Affiliation:
Departamento de Ecología Evolutiva, Museo Nacional de Ciencias Naturales, MNCN-CSIC, José Gutiérrez Abascal, 2, 28006, Madrid, Spain Unidad Asociada de Ecología e Inmunología parasitaria, CSIC-Universidad de Murcia, Spain Departamento de Ecología Funcional y Evolutiva, Estación Experimental de Zonas Áridas, EEZA-CSIC, Ctra. de Sacramento s/n, La Cañada de San Urbano, 04120, Almería, Spain
Corresponding
E-mail address:

Abstract

Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2015 

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Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
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