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A molecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador

Published online by Cambridge University Press:  22 February 2007

M. Iwagami
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan:
C. Monroy
Affiliation:
Department of Biological Science, San Carlos University, Guatemala City, Guatemala:
M.A. Rosas
Affiliation:
Department of Biological Science, San Carlos University, Guatemala City, Guatemala:
M.R. Pinto
Affiliation:
Faculty of Medical Science, San Carlos University, Guatemala City, Guatemala:
A.G. Guevara
Affiliation:
Laboratory of Clinical Investigations, Hospital Vozandes, Quito, Ecuador:
J.C. Vieira
Affiliation:
Laboratory of Clinical Investigations, Hospital Vozandes, Quito, Ecuador:
Y. Agatsuma
Affiliation:
Laboratory of Clinical Technology, Kochi Gakuen College, Kochi 780-0955, Japan
T. Agatsuma*
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan:
*
*Author for correspondence Fax: +81 88 880 2535 Email: agatsuma@med.kochi-ms.ac.jp

Abstract

A molecular phylogeographic study of Paragonimus mexicanus collected from Guatemala and Ecuador was performed. Genomic DNA was extracted from individual metacercariae, and two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified by the polymerase chain reaction (PCR). Sequences segregated in a phylogenetic tree according to their geographic origins. ITS2 sequences from Ecuador and Guatemala differed at only one site. Pairwise distances among CO1 sequences within a country were always lower than between countries. Nevertheless, genetic distances between countries were less than between geographical forms of P. westermani that have been suggested to be distinct species. This result suggests that populations from Guatemala and Ecuador are genetically differentiated perhaps at the level of subspecies.

Type
Review Article
Copyright
Cambridge University Press 2003

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A molecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador
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