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Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran

Published online by Cambridge University Press:  08 October 2013

S. Rostami
Affiliation:
Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman76169-14111, Iran
R. Salavati
Affiliation:
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada
R.N. Beech
Affiliation:
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada
Z. Babaei
Affiliation:
Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman76169-14111, Iran
M. Sharbatkhori
Affiliation:
Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
M.R. Baneshi
Affiliation:
Research Center for Modeling in Health, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
E. Hajialilo
Affiliation:
Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
H. Shad
Affiliation:
Iranian Veterinary Organization, Shahr-e-Ray, Tehran, Iran
M.F. Harandi
Affiliation:
Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman76169-14111, Iran
Corresponding
E-mail address:

Abstract

Although Taenia hydatigena is one of the most prevalent taeniid species of livestock, very little molecular genetic information exists for this parasite. Up to 100 sheep isolates of T. hydatigena were collected from 19 abattoirs located in the provinces of Tehran, Alborz and Kerman. A calibrated microscope was used to measure the larval rostellar hook lengths. Following DNA extraction, fragments of cytochrome c oxidase 1 (CO1) and 12S rRNA genes were amplified by the polymerase chain reaction method and the amplicons were subjected to sequencing. The mean total length of large and small hooks was 203.4 μm and 135.9 μm, respectively. Forty CO1 and 39 12S rRNA sequence haplotypes were obtained in the study. The levels of pairwise nucleotide variation between individual haplotypes of CO1 and 12S rRNA genes were determined to be between 0.3–3.4% and 0.2–2.1%, respectively. The overall nucleotide variation among all the CO1 haplotypes was 9.7%, and for all the 12S rRNA haplotypes it was 10.1%. A significant difference was observed between rostellar hook morphometry and both CO1 and 12S rRNA sequence variability. A significantly high level of genetic variation was observed in the present study. The results showed that the 12S rRNA gene is more variable than CO1.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2013 

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References

Abidi, S.M.A., Nizami, W.A., Khan, P., Ahmad, M. & Irshadullah, M. (1989) Biochemical characterization of Taenia hydatigena cysticerci from goats and pigs. Journal of Helminthology 63, 333337.CrossRefGoogle Scholar
Avise, J.C. (2000) Phylogeography: the history and formation of species. Harvard, Harvard University Press.Google Scholar
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165173.CrossRefGoogle ScholarPubMed
Campbell, G., Garcia, H.H., Nakao, M., Ito, A. & Craig, P.S. (2006) Genetic variation in Taenia solium. Parasitology International 55, S121S126.CrossRefGoogle Scholar
Dalimi, A., Sattari, A. & Motamedi, G.H. (2006) A study on intestinal helminthes of dogs, foxes and jackals in the western part of Iran. Veterinary Parasitology 142, 129133.CrossRefGoogle ScholarPubMed
Edwards, G.T. & Herbert, I.V. (1981) Some quantitative characters used in the identification of Taenia hydatigena, T. ovis, T. pisiformis. Journal of Helminthology 55, 17.CrossRefGoogle Scholar
Fakae, B.B. (1990) The epidemiology of helminthosis in small ruminants under the traditional husbandry system in eastern Nigeria. Veterinary Research Communications 14, 381391.CrossRefGoogle ScholarPubMed
Gasser, R.B., Zhu, X. & McManus, D.P. (1999) NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda) 1. International Journal for Parasitology 29, 19651970.CrossRefGoogle ScholarPubMed
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 9598.Google Scholar
Harandi, M.F., Hobbs, R.P., Adams, P.J., Mobedi, I., Morgan-Ryan, U.M. & Thompson, R.C.A. (2002) Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 125, 367373.Google ScholarPubMed
Harandi, M.F., Moazezi, S.S., Saba, M., Grimm, F., Kamyabi, H., Sheikhzadeh, F., Sharifi, I. & Deplazes, P. (2010) Sonographical and serological survey of human cystic echinococcosis and analysis of risk factors associated with seroconversion in rural communities of Kerman, Iran. Zoonoses and Public Health 58, 582588.CrossRefGoogle Scholar
Hasslinger, M.A. & Weber-Werringhen, R. (1988) Fecal surveys in pastured sheep and the occurrence of Cysticercus tenuicollis in slaughtered sheep. Angewandte Parasitologie 29, 227.Google Scholar
Hejazi, S.H., Pestehchian, N. & Abdi, J. (2004) A study of stray dog cestodes in Isfahan. Journal of Isfahan Medical School (IUMS) 22, 5053.Google Scholar
Hosseini, S.H. & Habibi, M. (2000) Gastrointestinal helminthes of sheepdog in Ardestan (Isfahan province – Iran). Pajouhesh-va-Sazandegi 108109.Google Scholar
Hustead, S.T. & Williams, J.F. (1977) Permeability studies on taeniid metacestodes: I. Uptake of proteins by larval stages of Taenia taeniaeformis, T. crassiceps, and Echinococcus granulosus. Journal of Parasitology 63, 314321.CrossRefGoogle Scholar
Jepson, P.G. & Hinton, M.H. (1986) An inquiry into the causes of liver damage in lambs. Veterinary Record 118, 584587.CrossRefGoogle Scholar
Jia, W.-Z., Yan, H.-B., Guo, A.-J., Zhu, X.-Q., Wang, Y.-C., Shi, W.-G., Chen, H.-T., Zhan, F., Zhang, S.-H. & Fu, B.-Q. (2010) Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 11, 447.CrossRefGoogle Scholar
Kedra, A.H., Tkach, V.V., Swiderski, Z. & Pawlowski, Z. (2001) Intraspecific variability among NADH dehydrogenase subunit 1 sequences of Taenia hydatigena. Parasitology International 50, 145148.CrossRefGoogle ScholarPubMed
Lavikainen, A., Haukisalmi, V., Lehtinen, M.J., Henttonen, H., Oksanen, A. & Meri, S. (2008) A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 135, 14571467.CrossRefGoogle ScholarPubMed
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 14511452.CrossRefGoogle ScholarPubMed
Loos-Frank, B. (2000) An up-date of Verster's (1969) Taxonomic revision of the genus Taenia Linnaeus (Cestoda) in table format. Systematic Parasitology 45, 155184.CrossRefGoogle Scholar
McManus, D.P. (2002) The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 151157.CrossRefGoogle ScholarPubMed
McManus, D.P. & Bowles, J. (1994) Asian (Taiwan) Taenia: species or strain? Parasitology Today 10, 273275.CrossRefGoogle ScholarPubMed
Mills, G.L., Coley, S.C. & Williams, J.F. (1983) Chemical composition of lipid droplets isolated from larvae of Taenia taeniaeformis. Journal of Parasitology 69, 850856.CrossRefGoogle ScholarPubMed
Moritz, C., Dowling, T.E. & Brown, W.M. (1987) Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Annual Review of Ecology and Systematics 18, 269292.CrossRefGoogle Scholar
Murat, K. (2005) Investigation of antigenic specificity against Cysticercus tenuicollis cyst fluid antigen in dogs experimentally infected with Taenia hydatigena. Turkish Journal of Veterinary Animal Sciences 29, 835840.Google Scholar
Murrell, K.D., Dorny, P., Flisser, A., Geerts, S., Kyvsgaard, N.C., McManus, D.P. & Nash, T.E. (2005) WHO/FAO/OIE Guidelines for the surveillance, prevention and control of taeniosis/cysticercosis. 156 pp.Paris, OIE/WHO/FAO.Google Scholar
Nwosu, C.O., Ogunrinade, A.F. & Fagbemi, B.O. (1996) Prevalence and seasonal changes in the gastro-intestinal helminths of Nigerian goats. Journal of Helminthology 70, 329333.CrossRefGoogle ScholarPubMed
Okamoto, M., Ito, A., Kurosawa, T., Oku, Y., Kamiya, M. & Agatsuma, T. (1995) Intraspecific variation of isoenzymes in Taenia taeniaeformis. International Journal for Parasitology 25, 221228.CrossRefGoogle ScholarPubMed
Okamoto, M., Agatsuma, T., Kurosawa, T. & Ito, A. (1997) Phylogenetic relationships of three hymenolepidid species inferred from nuclear ribosomal and mitochondrial DNA sequences. Parasitology 115, 661666.CrossRefGoogle ScholarPubMed
Page, R.D.M. (1996) TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357358.Google Scholar
Radfar, M.H., Tajalli, S. & Jalalzadeh, M. (2005) Prevalence and morphological characterization of Cysticercus tenuicollis (Taenia hydatigena cysticerci) from sheep and goats in Iran. Veterinarski arhiv 75, 469476.Google Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F.D.R., Jeanmougin, F.O. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.CrossRefGoogle Scholar
Utuk, A.E. & Piskin, F.C. (2012) Molecular detection and characterization of goat isolate of Taenia hydatigena in Turkey. The Scientific World Journal doi:10.1100/2012/962732.CrossRefGoogle ScholarPubMed
Varcasia, A., Tanda, B., Giobbe, M., Solinas, C., Pipia, A.P., Malgor, R., Carmona, C., Garippa, G. & Scala, A. (2011) Cystic echinococcosis in Sardinia: farmers' knowledge and dog infection in sheep farms. Veterinary Parasitology 181, 335340.CrossRefGoogle ScholarPubMed
Verster, A. (1969) A taxonomic revision of the genus Taenia Linnaeus. Onderstepoort Journal of Veterinary Research 36, 358.Google Scholar
von Nickisch-Rosenegk, M., Lucius, R. & Loos-Frank, B. (1999) Contributions to the phylogeny of the Cyclophyllidea (Cestoda) inferred from mitochondrial 12S rDNA. Journal of Molecular Evolution 48, 586596.CrossRefGoogle ScholarPubMed
Wondimu, A., Abera, D. & Hailu, Y. (2011) A study on the prevalence, distribution and economic importance of Cysticercus tenuicollis in visceral organs of small ruminants slaughtered at an abattoir in Ethiopia. Journal of Veterinary Medicine and Animal Health 3, 6774.Google Scholar
Zhang, L., Hu, M., Jones, A., Allsopp, B.A., Beveridge, I., Schindler, A.R. & Gasser, R.B. (2007) Characterization of Taenia madoquae and Taenia regis from carnivores in Kenya using genetic markers in nuclear and mitochondrial DNA, and their relationships with other selected taeniids. Molecular and Cellular Probes 21, 379385.CrossRefGoogle Scholar
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Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran
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