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Identities of two Paragonimus species from Sri Lanka inferred from molecular sequences

Published online by Cambridge University Press:  22 February 2007

M. Iwagami
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
R.P.V.J. Rajapakse
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Science, University of Peradeniya, Peradeniya, Sri Lanka
W. Paranagama
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Science, University of Peradeniya, Peradeniya, Sri Lanka
T. Agatsuma*
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
*Corresponding
*Author for correspondence Fax: +81 88 880 2535 E-mail: agatsuma@med.kochi-ms.ac.jp

Abstract

Metacercariae of Paragonimus spp. were obtained from field-collected freshwater crabs in Sri Lanka. Genomic DNA was extracted from single metacercariae. Two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified using the polymerase chain reaction. Two differing sequences were obtained for each of these gene regions. Phylogenetic analyses placed the type 1 sequences as sister to a clade containing P. westermani and P. siamensis whereas the type 2 sequences were close to published sequences of P. siamensis from Thailand. The possible taxonomic status of these two types are discussed. This is the first report of molecular data about Paragonimus from Sri Lanka.

Type
Review Article
Copyright
Cambridge University Press 2003

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