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Protective effect of maternal exercise against amyloid-β neurotoxicity in the male rat offspring’s cerebellum

Part of: DOHaD Research in Latin-American

Published online by Cambridge University Press:  23 June 2020

Caroline Peres Klein ,
Juliana Bender Hoppe ,
André Brum Saccomori ,
Bernardo Gindri dos Santos Open the ORCID record for Bernardo Gindri dos Santos [Opens in a new window] ,
Pauline Maciel August ,
Isadora Peres Klein ,
Mariana Scortegagna Crestani ,
Felippo Bifi ,
Régis Mateus Hözer  and
Plácido Navas
...Show all authors
Caroline Peres Klein
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Juliana Bender Hoppe
Affiliation:
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
André Brum Saccomori
Affiliation:
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Bernardo Gindri dos Santos
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Pauline Maciel August
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Isadora Peres Klein
Affiliation:
Programa de Pós-Graduação em Patologia Oral, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Mariana Scortegagna Crestani
Affiliation:
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Felippo Bifi
Affiliation:
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Régis Mateus Hözer
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Plácido Navas
Affiliation:
Centro Andaluz de Biología del Desarrollo and CIBERER, Instituto de Salud Carlos III, Universidad Pablo de Olavide-CSIC-JA, 41013Sevilla, Spain
Christianne Gazzana Salbego
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
Cristiane Matté*
Affiliation:
Programa de Pós-Graduação em Ciências Biológicas – Bioquímica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil Programa de Pós-Graduação em Ciências Biológicas – Fisiologia, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
*
Address for correspondence: Cristiane Matté, Rua Ramiro Barcelos, 2600-Anexo, Porto Alegre Zip Code: 90035-003, RS, Brazil. Email: matte@ufrgs.br
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Abstract

The Developmental Origins of Health and Disease (DOHaD) states that intrauterine maternal environment influences postnatal life by programming offspring’s metabolism. Intrauterine milieu induced by exercise during pregnancy promotes long-lasting benefits to the offspring’s health and seems to offer some resistance against chronic diseases in adult life. Alzheimer’s disease is a public health concern with limited treatment options. In the present study, we assessed the potential of maternal exercise during pregnancy in long-term programming of young adult male rat offspring’s cerebellar metabolism in conferring neuroprotection against amyloid-β (Aβ) neurotoxicity. Female Wistar rats were submitted to a swimming protocol 1 week prior mating and throughout pregnancy (five sessions/a week lasting 30 min). Aβ oligomers were infused bilaterally in the brain ventricles of 60-day-old male offspring. Fourteen days after surgery, we measured parameters related to redox state, mitochondrial function, and the immunocontent of proteins related to synaptic function. We found that maternal exercise during pregnancy attenuated several parameters in the offspring’s male rat cerebellum, such as the reactive species rise, the increase of inducible nitric oxide synthase immunocontent and tau phosphorylation induced by Aβ oligomers, increased mitochondrial fission indicated by dynamin-related protein 1 (DRP1), and protein oxidation identified by carbonylation. Strikingly, we find that maternal exercise promotes changes in the rat offspring’s cerebellum that are still evident in young adult life. These favorable neurochemical changes in offspring’s cerebellum induced by maternal exercise may contribute to a protective phenotype against Aβ-induced neurotoxicity in young adult male rat offspring.

Keywords

Intrauterine metabolic programmingDOHADexercisebioenergeticsAlzheimer’s disease
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 11 , Issue 5 , October 2020 , pp. 521 - 532
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2020

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