Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-07-03T10:14:43.328Z Has data issue: false hasContentIssue false
  • English
  • Français

Synthesis of plasminogen activator inhibitor 1 by bovine mammary epithelial and myoepithelial cell lines

Published online by Cambridge University Press:  01 June 2009

Boris Zavizion ,
Christian W. Heegard ,
Jeffrey White ,
Federica Cheli  and
Ioannis Politis
Boris Zavizion
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05404, USA
Christian W. Heegard
Affiliation:
Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark
Jeffrey White
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05404, USA
Federica Cheli
Affiliation:
Istituto di Nutrizione Animale, Università degli Studi di Milano, I-20133 Milano, Italia
Ioannis Politis
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05404, USA
Get access Rights & Permissions [Opens in a new window]

Summary

Conversion of plasminogen to plasmin provides an important source of proteolytic activity in the bovine mammary gland. Plasminogen activator inhibitor 1 (PAI-1) plays a key role in limiting plasminogen activation. The PAI-1 biosynthetic capabilities of various bovine mammary cells were determined. The immortalized epithelial cell lines MAC-T and BME-UV and the myoepithelial cell line BMM-UV were used as model systems. Northern blot analysis indicated that both epithelial and myoepithelial cells contained PAI-1 mRNA. Bovine PAI-1 was encoded by a single mRNA species ∼ 3·0 kb long. BME-UV cells contained 2·0-fold (P < 0·01) the PAI-1 mRNA of MAC-T or BMM-UV cells. Reverse zymography indicated that both epithelial and myoepithelial cells synthesized PAI-1 protein with a molecular mass of ∼ 50 kDa.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 3 , August 1996 , pp. 451 - 458
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andersson, L. M., Dundas, S. R., O'Hare, M. J., Gusterson, B. A. & Warburton, M. J. 1994 Synthesis of gelatinases by rat mammary epithelial and myoepithelial cell lines. Experimental Cell Research 212 389–392CrossRefGoogle ScholarPubMed
Andreasen, P. A., George, B., Lund, L. R., Riccio, A. & Stacey, S. N. 1990 Plasminogen activator inhibitors: horinonally regulated serpins. Molecular and Cellular Endocrinology 68 1–19CrossRefGoogle ScholarPubMed
Andreasen, P. A., Sottrup-Jensen, L., Kjoller, L., Nykjaer, A., Moestrup, S. K., Petersen, C. M. & Gliemann, J. 1994 Receptor-mediated endocytosis of plasminogen activators and activator/inhibitor complexes. FEBS Letters 338 239–245CrossRefGoogle ScholarPubMed
Chomczynski, P. & Sacchi, N. 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162 156–159CrossRefGoogle ScholarPubMed
Dickson, S. R. &, Warburton, M. J. 1992 Enhanced synthesis of gelatinase and stromelysin by mammary cells during involution of the rat mammary gland. Journal of Histochemistry and Cytochemistry 40 697–703CrossRefGoogle ScholarPubMed
Ellis, V., Behrendt, N. & Dano, R. 1991 Plasminogen activation by receptor-bound urokinase. Thrombosis and Haemostasis 65 683Google Scholar
Hasselaar, P., Loskutoff, D. J., Sawdey, M. & Sage, E. H. 1991 SPARC induces the expression of type 1 plasminogen activator inhibitor in cultured bovine aortic endothelial cells. Journal of Biological Chemistry 266 13178–13184CrossRefGoogle ScholarPubMed
Heegaard, C. W., Rasmussen, L. K. & Andreasen, P. A. 1994 The plasminogen activation system in bovine milk: differential localization of tissue-type plasminogen activator and urokinase in milk fractions is caused by binding to casein and urokinase receptor. Biochimica et Biophysica Ada 1222 45–55CrossRefGoogle ScholarPubMed
Heegard, C. E., White, J. H., Zavizion, B., Turner, J. D. & Politis, I. 1994 Production of various forms of plasminogen activator and plasminogen activator inhibitor by cultured mammary epithelial cells. Journal of Dairy Science 77 2949–2958CrossRefGoogle ScholarPubMed
Huynh, H. T., Robitaille, G. & Turner, J. D. 1991 Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Experimental Cell Research 197 191–199CrossRefGoogle Scholar
Ossowski, L., Biegel, D. & Reich, E. 1979 Mammary plasminogen activator: correlation with involution, hormonal modulation and comparison between normal and neoplastic tissue. Cell 16 929–940CrossRefGoogle ScholarPubMed
Pepper, M. S., Belin, D., Montesano, R., Orci, L. & Vassalli, J. -D. 1990 Transforming growth factor-beta 1 modulates basic flbroblast growth factor-induced proteolytic and angiogenic properties of endothelial cells in vitro. Journal of Cell Biology 111 743–755CrossRefGoogle Scholar
Politis, I. 1996 Plasminogen activator system: implications for mammary cell growth and involution. Journal of Dairy Science (In press)CrossRefGoogle ScholarPubMed
Politis, I., Barbano, D. M. & Gorewit, R. C. 1992 Changes in plasminogen activator in different physiological states of the gland. Journal of Dairy Science 75 Suppl. 1 181 (Abstr.)Google Scholar
Politis, I., Lachance, E., Block, E. & Turner, J. D. 1989 a Plasmin and plasminogen in bovine milk: a relationship with involution? Journal of Dairy Science 72 900–906CrossRefGoogle ScholarPubMed
Politis, I., Ng Kwai Hang, K. F. & Giroux, R. N. 1989 b Environmental factors affecting plasmin activity in milk. Journal of Dairy Science 72 1713–1718CrossRefGoogle ScholarPubMed
Politis, I., Zavizion, B., White, J. H., Goldberg, J. J., Baldi, A. & Akers, R. M. 1995 Hormonal and extracellular matrix regulation of plasminogen activator in a bovine mammary epithelial cell line. Endocrine 3 345–350CrossRefGoogle Scholar
Saksela, O. & Rifkin, D. B. 1988 Cell-associated plasminogen activation: regulation and physiological functions. Annual Review of Cell Biology 4 93–126CrossRefGoogle ScholarPubMed
Sprengers, E. D. & Kluft, C. 1987 Plasminogen activator inhibitors. Brief review. Blood 69 381–387CrossRefGoogle Scholar
Zavizion, B., van Duffelen, M., Schaeffbr, W. & Politis, I. 1996 a Establishment and characterization of a bovine mammary myoepithelial cell line. In Vitro Cellular and Developmental Biology 32 149–158CrossRefGoogle ScholarPubMed
Zavizion, B., van Duffelen, M., Schaeffer, W. & Politis, I. 1996 b Establishment and characterization of a bovine mammary epithelial cell line with unique properties. In Vitro Cellular and Developmental Biology 32 138–148CrossRefGoogle ScholarPubMed