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Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

Published online by Cambridge University Press:  01 February 1997

CHRISTOPHER M. GOODING  and
PRABHAKARA V. CHOUDARY
CHRISTOPHER M. GOODING
Affiliation:
CIFAR, Antibody Engineering Laboratory and Department of Entomology, University of California, Davis, CA 95616-8584, USA
PRABHAKARA V. CHOUDARY
Affiliation:
CIFAR, Antibody Engineering Laboratory and Department of Entomology, University of California, Davis, CA 95616-8584, USA
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Abstract

Escherichia coli O157[ratio ]H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157[ratio ]H7/g food could be detected in <10 h.

Type
Research Article
Information
Journal of Dairy Research , Volume 64 , Issue 1 , February 1997 , pp. 87 - 93
Copyright
Proprietors of Journal of Dairy Research 1997

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