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Purification of goats' milk casein by reversed-phase high-performance liquid chromatography and identification of αs1-casein

Published online by Cambridge University Press:  01 June 2009

Jan Mikkelsen ,
Peter Højrup  and
Jens Knudsen
Jan Mikkelsen
Affiliation:
Institute of Biochemistry, DK-5230 Odense M, Denmark
Peter Højrup
Affiliation:
Institute of Molecular Biology, University of Odense, DK-5230 Odense M, Denmark
Jens Knudsen
Affiliation:
Institute of Biochemistry, DK-5230 Odense M, Denmark
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Summary

Goats’ milk caseins were separated into four components in a single run using reversed-phase gradient high-performance liquid chromatography. The purity of the isolated components was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis, amino acid analysis and determination of the N-terminal residue. By a comparison with previously published results for goats’ milk caseins the four peaks were identified as κ-, αs1-, αs2- and β-casein. In order to confirm the existence of αsl-casein in goats’ milk, this component was sequenced for 44 steps, revealing a sequence homologous to bovine αsl-casein and almost identical to the N-terminal sequence previously published by Boulanger et al. (1984).

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 3 , August 1987 , pp. 361 - 367
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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