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Inhibition of mitogen-induced proliferative responses of lymphocytes by bovine κ-caseinoglycopeptides having different carbohydrate chains

Published online by Cambridge University Press:  01 June 2009

Hajime Otani ,
Makoto Monnai ,
Yoshihiro Kawasaki ,
Hiroshi Kawakami  and
Morimasa Tanimoto
Hajime Otani
Affiliation:
Laboratory of Applied Biochemistry of Animal Products, Faculty of Agriculture, Shinshu University, Minamiminowa-mura 399-45, Japan
Makoto Monnai
Affiliation:
Laboratory of Applied Biochemistry of Animal Products, Faculty of Agriculture, Shinshu University, Minamiminowa-mura 399-45, Japan
Yoshihiro Kawasaki
Affiliation:
Laboratory of Applied Biochemistry of Animal Products, Faculty of Agriculture, Shinshu University, Minamiminowa-mura 399-45, Japan Technical Research Institute, Snow Brand Milk Products Co. Ltd, Minamidai, Kawagoe 350, Japan
Hiroshi Kawakami
Affiliation:
Laboratory of Applied Biochemistry of Animal Products, Faculty of Agriculture, Shinshu University, Minamiminowa-mura 399-45, Japan Technical Research Institute, Snow Brand Milk Products Co. Ltd, Minamidai, Kawagoe 350, Japan
Morimasa Tanimoto
Affiliation:
Laboratory of Applied Biochemistry of Animal Products, Faculty of Agriculture, Shinshu University, Minamiminowa-mura 399-45, Japan Technical Research Institute, Snow Brand Milk Products Co. Ltd, Minamidai, Kawagoe 350, Japan
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Summary

Bovine κ-caseinoglycopeptides (i.e. residues 106–169, CGP) were prepared from κ-casein digested with rennin and a commercial whey protein concentrate. CGP from whey protein concentrate was further divided into seven CGP fractions having different carbohydrate compositions using FPLC. Unfractionated CGP inhibited lipopolysaccharide (LPS)- and phytohaemagglutinin (PHA)-induced proliferative responses of mouse spleen cells and rabbit Peyer's patch cells. The unfractionated CGP also inhibited antibody responses to sheep red blood cells in mouse spleen cell cultures. However, seven CGP fractions having zero to five N-acetylneuraminic acid (NANA) residues had different inhibitory effects on both LPS- and PHA-induced proliferative responses of mouse spleen cells. The inhibitory effect on PHA-induced proliferative responses increased with increasing numbers of NANA residues, whereas that on LPS-induced proliferation was highest with the CGP fraction having two NANA residues. Both inhibitory effects decreased significantly after neuraminidase or chymotrypsin digestion. These findings indicate that both the carbohydrate (particularly the NANA residues) and the polypeptide portions are essential for inhibitory effects on LPS- and PHA-induced proliferative responses of mouse spleen cells.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 2 , May 1995 , pp. 349 - 357
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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