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Going further post-RNA-seq: In silico functional analyses revealing candidate genes and regulatory elements related to mastitis in dairy cattle

Published online by Cambridge University Press:  10 August 2021

Hyago Passe Pereira ,
Lucas Lima Verardo ,
Mayara Morena Del Cambre Amaral Weller ,
Ana Paula Sbardella ,
Danísio Prado Munari ,
Raquel Morais de Paiva Daibert ,
Wanessa Araújo Carvalho ,
Marco Antonio Machado  and
Marta Fonseca Martins
Hyago Passe Pereira
Affiliation:
Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Lucas Lima Verardo
Affiliation:
Zootechnics Department, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazil
Mayara Morena Del Cambre Amaral Weller
Affiliation:
Zootechnics Department, Universidade Federal do Espírito Santo, Alegre, Brazil
Ana Paula Sbardella
Affiliation:
Department of Exact Sciences, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, Brazil
Danísio Prado Munari
Affiliation:
Department of Exact Sciences, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, Brazil
Raquel Morais de Paiva Daibert
Affiliation:
Molecular Genetics Laboratory, Embrapa Gado de Leite, Juiz de Fora, Brazil
Wanessa Araújo Carvalho
Affiliation:
Molecular Genetics Laboratory, Embrapa Gado de Leite, Juiz de Fora, Brazil
Marco Antonio Machado
Affiliation:
Molecular Genetics Laboratory, Embrapa Gado de Leite, Juiz de Fora, Brazil
Marta Fonseca Martins*
Affiliation:
Molecular Genetics Laboratory, Embrapa Gado de Leite, Juiz de Fora, Brazil
*
Author for correspondence: Marta Fonseca Martins, Email: marta.martins@embrapa.br
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Abstract

This study aimed to obtain a better understanding of the regulatory genes and molecules involved in the development of mastitis. For this purpose, the transcription factors (TF) and MicroRNAs (miRNA) related to differentially expressed genes previously found in extracorporeal udders infected with Streptococcus agalactiae were investigated. The Gene-TF network highlighted LOC515333, SAA3, CD14, NFKBIA, APOC2 and LOC100335608 and genes that encode the most representative transcription factors STAT3, PPARG, EGR1 and NFKB1 for infected udders. In addition, it was possible to highlight, through the analysis of the gene-miRNA network, genes that could be post-transcriptionally regulated by miRNAs, such as the relationship between the CCL5 gene and the miRNA bta-miR-363. Overall, our data demonstrated genes and regulatory elements (TF and miRNA) that can play an important role in mastitis resistance. The results provide new insights into the first functional pathways and the network of genes that orchestrate the innate immune responses to infection by Streptococcus agalactiae. Our results will increase the general knowledge about the gene networks, transcription factors and miRNAs involved in fighting intramammary infection and maintaining tissue during infection and thus enable a better understanding of the pathophysiology of mastitis.

Keywords

Bovineextracorporeal uddersmastitisMicroRNAtranscription factors
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 286 - 292
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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