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Functional analysis of the dairy cow mammary transcriptome between early lactation and mid-dry period

Published online by Cambridge University Press:  07 February 2019

Ye Lin ,
He Lv ,
Minghui Jiang ,
Jinyu Zhou ,
Shuyuan Song  and
Xiaoming Hou
Ye Lin
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
He Lv
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Minghui Jiang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Jinyu Zhou
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Shuyuan Song
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Xiaoming Hou*
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
*
Author for correspondence: Xiaoming Hou, Email: xiaominghou@126.com
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Abstract

In this research communication we used digital gene expression (DGE) analysis to identify differences in gene expression in the mammary glands of dairy cows between early lactation and the mid-dry period. A total of 741 genes were identified as being differentially expressed by DGE analysis. Compared with their expression in dry cows, 214 genes were up-regulated and 527 genes were down-regulated in lactating cow mammary glands. Gene Ontology analysis showed that lactation was supported by increased gene expression related to metabolic processes and nutrient transport and was associated with decreased gene expression related to cell proliferation. Pathway mapping using the Kyoto Encyclopedia of Genes and Genomes showed that 579 differentially expressed genes had pathway annotations related to 204 pathways. Metabolic pathway-related genes were the most significantly enriched. Genes and pathways identified by the present study provide insights into molecular events that occur in the mammary gland between early lactation and mid-dry period, which can be used to facilitate further investigation of the mechanisms underlying lactation and mammary tissue remodeling in dairy cows.

Keywords

Differential gene expressiondry periodlactationmammary gland
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 63 - 67
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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References

Bionaz, M and Loor, JJ (2008) Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9, 366.Google Scholar
Bionaz, M and Loor, JJ (2011) Gene networks driving bovine mammary protein synthesis during the lactation cycle. Bioinformatics and Biology Insights 5, 83–98.Google Scholar
Capuco, AV, Akers, RM and Smith, JJ (1997) Mammary growth in Holstein cows during the dry period: quantification of nucleic acids and histology. Journal of Dairy Science 80, 477–487.Google Scholar
Collier, RJ, Annen-Dawson, EL and Pezeshki, A (2012) Effects of continuous lactation and short dry periods on mammary function and animal health. Animal 6, 403–414.Google Scholar
Dado-Senn, B, Skibiel, AL, Fabris, TF, Zhang, Y, Dahl, GE, Penagaricano, F and Laporta, J (2018) RNA-Seq reveals novel genes and pathways involved in bovine mammary involution during the dry period and under environmental heat stress. Scientific Reports 8, 11096.Google Scholar
Gao, Y, Lin, X, Shi, K, Yan, Z and Wang, Z (2013) Bovine mammary gene expression profiling during the onset of lactation. PLoS One 8, e70393.Google Scholar
Sinowatz, F, Schams, D, Habermann, F, Berisha, B and Vermehren, M (2006) Localization of fibroblast growth factor I (acid fibroblast growth factor) and its mRNA in the bovine mammary gland during mammogenesis, lactation and involution. Anatomia Histologia Embryologia 35, 202–207.Google Scholar
‘t Hoen, PA, Ariyurek, Y, Thygesen, HH, Vreugdenhil, E, Vossen, RH, de Menezes, RX, Boer, JM, van Ommen, GJ and den Dunnen, JT (2008) Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray platforms. Nucleic Acids Research 36, e141.Google Scholar
Truchet, S, Chat, S and Ollivier-Bousquet, M (2014) Milk secretion: the role of SNARE proteins. Journal of Mammary Gland Biology and Neoplasia 19, 119–130.Google Scholar
Wang, W, Lv, N, Zhang, S, Shui, G, Qian, H, Zhang, J, Chen, Y, Ye, J, Xie, Y, Shen, Y, Wenk, MR and Li, P (2012) Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids. Nature Medicine 18, 235–243.Google Scholar
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