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Effects of prolonged treatment of lactating goats with bovine somatotropin on aspects of adipose tissue and liver metabolism

Published online by Cambridge University Press:  01 June 2009

Richard G. Vernon ,
Anne Faulkner ,
Eric Finley ,
Paul W. Watt  and
Victor A. Zammit
Richard G. Vernon
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
Anne Faulkner
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
Eric Finley
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
Paul W. Watt
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
Victor A. Zammit
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

The effects of prolonged (22 weeks) treatment of lactating goats with bovine somatotropin on the metabolism of adipose tissue and liver has been investigated. Somatotropin treatment resulted in smaller adipocytes, decreased rate of fatty acid synthesis and decreased total acetyl-CoA carboxylase activity of adipocytes, but with no change in the proportion of this enzyme in the active state. The rate of acylglycerol glycerol synthesis from glucose of adipocytes tended to decrease as did total glucose utilization by the tissue. Glucose conversion to lactate was unchanged by somatotropin treatment but glucose conversion to other products was decreased. Maximum response of adipose tissue to insulin was unchanged but the sensitivity to insulin decreased on somatotropin treatment. Treatment with somatotropin had no effect on basal lipolysis and decreased maximum response to the β-agonist isoproterenol, but this probably reflects the rate of isoproterenol-stimulated lipolysis varying with cell volume in adipocytes. No apparent change in response either to α2-adrenergic agonists or to adenosine was apparent. The number of β-adrenergic receptors was unchanged in adipocyte membranes but the number of α2-adrenergic receptors increased. The rate of hepatic gluconeogenesis in vitro, the activity of key gluconeogenic enzymes and the modulation of the rate of gluconeogenesis by butyrate were unchanged except for the effect of this latter agent on gluconeogenesis from propionate. Hepatic ketogenic activity, as indicated by the activity of carnitine palmitoyl-CoA-transferase-1 and the concentrations of carnitine and acyl carnitines, was unchanged by treatment. Thus at the end of a prolonged period of treatment with somatotropin in lactating goats, lipid synthesis in adipose tissue is still decreased but no effects on liver lipid and carbohydrate metabolism were apparent.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 2 , May 1995 , pp. 237 - 248
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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