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Different chronological patterns of appearance of blood derived milk components during mastitis indicate different mechanisms of transfer from blood into milk

Published online by Cambridge University Press:  03 July 2015

Olga Wellnitz ,
Christina Zbinden ,
Johannes Lüttgenau ,
Heinrich Bollwein  and
Rupert M Bruckmaier
Olga Wellnitz
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Christina Zbinden
Affiliation:
Vetsuisse Faculty University of Bern, Veterinary Physiology, Switzerland
Johannes Lüttgenau
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Heinrich Bollwein
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Rupert M Bruckmaier*
Affiliation:
Vetsuisse Faculty University of Bern, Veterinary Physiology, Switzerland
*
*For correspondence; e-mail: rupert.bruckmaier@vetsuisse.unibe.ch
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Abstract

This study aimed to describe chronological patterns of changes of various candidate blood components in milk during the acute phase of a mammary immune response in detail. Eight dairy cows were challenged with Escherichia coli lipopolysaccharide in one udder quarter. Milk from challenged and control quarters and blood samples were taken before, and 1 and 2 h after challenge and then every 15 min until 5 h after challenge. The SCC, serum albumin, immunoglobulin (Ig)G1, IgG2, lactate dehydrogenase (LDH), and L-lactate in milk and blood, and α-lactalbumin in blood were analysed. All selected parameters in milk increased in challenged quarters but did not increase in control quarters. Milk IgG1, IgG2, serum albumin, and LDH were already significantly increased at 2 h after challenge whereas a significant increase of SCC was detectable at 2·75 h and L-lactate was increased at 2·25 h after challenge. In blood L-lactate was increased at 3·75 h after challenge, however, other factors in blood did not change significantly within the 5 h of experiment. In conclusion, the increase of blood components in milk during inflammation follows two different patterns: There is a rapid increase for IgG1, IgG2, or LDH, before the increase of SCC, and their concentrations reach a plateau within 3 h. On the other hand, SCC and L-lactate show a slower but consistent increase not reaching a plateau within 5 h after LPS challenge. L-lactate increases to higher concentrations in milk than in blood. This clearly shows that the increase of blood components follows different patterns and is therefore a controlled and compound-specific process and not exclusively an unspecific type of leakage.

Keywords

dairy cowmastitisblood-milk barrier
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 322 - 327
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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