Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-23T13:15:47.797Z Has data issue: false hasContentIssue false
  • English
  • Français

DGAT1 polymorphism in Riverine buffalo, Swamp buffalo and crossbred buffalo

Published online by Cambridge University Press:  02 August 2018

Jun Li ,
Shenhe Liu ,
Zipeng Li ,
Shujun Zhang ,
Guohua Hua ,
Angela Salzano ,
Giuseppe Campanile ,
Bianca Gasparrini ,
Aixin Liang  and
Liguo Yang
Jun Li
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China Department of Immunology, Research Center for Medicine and Biology, Zunyi Medical University, Zunyi 563000, People's Republic of China
Shenhe Liu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Zipeng Li
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Shujun Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Guohua Hua
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Angela Salzano
Affiliation:
Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy
Giuseppe Campanile
Affiliation:
Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy
Bianca Gasparrini
Affiliation:
Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy
Aixin Liang*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Liguo Yang*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
*
*For correspondence; e-mail: yangliguo2006@qq.com, lax.pipi@mail.hzau.edu.cn
*For correspondence; e-mail: yangliguo2006@qq.com, lax.pipi@mail.hzau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

This Research Communication describes the polymorphisms in the coding region of DGAT1 gene in Riverine buffalo, Swamp buffalo and crossbred buffalo, and associations between polymorphisms and milk production performance in Riverine buffalo. Two polymorphisms of DGAT1were identified, located in exon 13 and exon 17, respectively. The distribution of the genotypes of the two SNP loci in different buffalo population varied, especially the polymorphism located in exon 13 which was not found in the Swamp buffalo. Moreover, SNP located in exon 17 was a nonsynonymous switch resulting in the animo acid sequence changed from an arginine (Arg) to a histidine (His) at position 484. Both SNPs were in Hardy–Weinberg equilibrium, and the polymorphism of g.8330T>C in the exon 13 was significantly associated with peak milk yield, total milk yield and protein percentage. The C variant was associated with an increase in milk yield and peak yield but less in protein percentage compared with the T variant. The polymorphisms of g.9046T>C in exon 17 were significantly associated with fat percentage, in that the buffaloes with TT genotype had a significantly higher fat percentage than those with CC genotype. These findings reveal the difference in the genetic evolution of the DGAT1 between Riverine buffalo and Swamp buffalo, and provide evidence that the DGAT1 gene has potential effects for Riverine buffalo milk production traits, which can be used as a candidate gene for marker-assisted selection in buffalo breeding.

Keywords

Buffalomilk productionDGAT1polymorphismassociation analysis
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 4 , November 2018 , pp. 412 - 415
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cases, S, Smith, SJ, Zheng, YW, Myers, HM, Lear, SR, Sande, E, Novak, S, Collins, C, Welch, CB, Lusis, AJ, Erickson, SK & Farese, RV 1998 Identification of a gene encoding an acyl CoA : diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proceedings of the National Academy of Sciences of the USA 95 13018–13023Google Scholar
Fang, L, Sahana, G, Su, G, Yu, Y, Zhang, S, Lund, MS & Rensen, P 2017 Integrating sequence-based GWAS and RNA-seq provides novel insights into the genetic basis of mastitis and milk production in dairy cattle. Scientific Reports 7 45560Google Scholar
Glazier, AM, Nadeau, JH & Aitman, TJ 2002 Finding genes that underlie complex traits. Science 298 2345–2349Google Scholar
Mishra, B, Tantia, MS, Kumar, STB & Vijh, RK 2007 Characterization of the DGAT1 gene in the Indian buffalo (Bubalus bubalis). Genetics and Molecular Biology 30 1097–1100Google Scholar
Naslund, J, Fikse, WF, Pielberg, GR & Lunden, A 2008 Frequency and effect of the bovine acyl-CoA : diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal of Dairy Science 91 2127–2134Google Scholar
Özdil, F & İlhan, F 2012 DGAT1 -exon8 polymorphism in Anatolian buffalo. Livestock Science 149 83–87Google Scholar
Smith, SJ, Cases, S, Jensen, DR, Chen, HC, Sande, E, Tow, B, Sanan, DA, Raber, J, Eckel, H & Farese, RV 2000 Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat. Nature Genetics 25 87–90Google Scholar
Spelman, RJ, Ford, CA, McElhinney, P, Gregory, GC & Snell, RG 2002 Characterization of the DGAT1 gene in the New Zealand dairy population. Journal of Dairy Science 85 3514–3517Google Scholar
Yuan, J, Zhou, J, Deng, X, Hu, X & Li, N 2007 Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene. Biochemical Genetics 45 611–621Google Scholar
Supplementary material: PDF

Li et al. supplementary material

Tables S1-S2

Download Li et al. supplementary material(PDF)
PDF 154.6 KB