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Determination of alkaline phosphatase activity in cheese using phenolphthalein monophosphate as substrate

Published online by Cambridge University Press:  01 June 2009

C. G. Rammell
Affiliation:
Wallaceville Dairy Laboratory, Department of Agriculture, Wellington, New Zealand
M. M. Joerin
Affiliation:
Wallaceville Dairy Laboratory, Department of Agriculture, Wellington, New Zealand

Summary

A method is described for determining alkaline phosphatase activity in cheese using phenolphthalein monophosphate as substrate. Results are read with a Lovibond comparator after 1 h incubation. Advisory standards are proposed based on a minimum pasteurization for 15 s at 71°C of the milk used for cheese-making.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 1972

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References

REFERENCES

Aschaffenburg, R. & Mullen, J. E. C. (1949). J. Dairy Res. 16, 58.CrossRefGoogle Scholar
Babson, A. L. (1965). Clin. Chem. 11, 789.CrossRefGoogle Scholar
Babson, A. L., Greeley, S. J., Coleman, C. M. & Phillips, G. E. (1966). Clin. Chem. 12, 482.CrossRefGoogle Scholar
Davis, J. G. (1955). A dictionary of dairying, 2nd edn.London: Leonard Hill Ltd.Google Scholar
Huggins, C. & Talalay, P. (1945). J. biol. Chem. 159, 399.CrossRefGoogle Scholar
Kleyn, D. H. & Lin, S. H. C. (1968). J. Ass. off. analyt. Chem. 51, 802.Google Scholar
McGugan, W. A. & Howsam, S. G. (1964). J. Dairy Sci. 47, 139.CrossRefGoogle Scholar
Rzhekhina, N. I. (1962). Biokhimiya 27, 359.Google Scholar