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Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections

Published online by Cambridge University Press:  05 May 2015

Norma B Russi ,
Julia Maito ,
Silvina A Dieser ,
María S Renna ,
Marcelo L Signorini ,
Cecilia Camussone ,
Verónica E Neder ,
Martín Pol ,
Liliana Tirante  and
Liliana M Odierno
...Show all authors
Norma B Russi
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Julia Maito
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Silvina A Dieser
Affiliation:
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km601, (5804) Río Cuarto, Córdoba, Argentina
María S Renna
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina
Marcelo L Signorini
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Cecilia Camussone
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Verónica E Neder
Affiliation:
Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Martín Pol
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Liliana Tirante
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Liliana M Odierno
Affiliation:
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km601, (5804) Río Cuarto, Córdoba, Argentina
Luis F Calvinho*
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
*
*For correspondence; e-mail: calvinho.luis@inta.gob.ar.
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Abstract

Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.

Keywords

Staphylococcus aureusintramammary infectionspenicillin resistanceblaZ
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 317 - 321
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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