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An evaluation of the Charm Test – a rapid method for the detection of penicillin in milk

Published online by Cambridge University Press:  01 June 2009

Shirley A Thorogood ,
Peter D. P. Wood  and
George A Prentice
Shirley A Thorogood
Affiliation:
Milk Marketing Board, Thames Dillon, Surrey KT7 OEL, UK
Peter D. P. Wood
Affiliation:
Milk Marketing Board, Thames Dillon, Surrey KT7 OEL, UK
George A Prentice
Affiliation:
Milk Marketing Board, Thames Dillon, Surrey KT7 OEL, UK
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Summary

Using the Charm radioactive assay technique as a rapid test for the determination of penicillin in milk in experiments with milks containing known concentrations of penicillin, the coefficient of variation was found to be 15 and 11% for raw whole and reconstituted skim-milks respectively. Storage of reagents for up to 4 d increased the variance of the test by more than 20% and the estimated penicillin concentration 0·4 %/d of storage up to 25d. In comparison with the Bromocresol Purple Test, which was capable of 100% accuracy in defining a threshold of 0·02 i.u. penicillin/ml milk, the Charm Test falsely classified 15·6% of the samples.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 2 , May 1983 , pp. 185 - 191
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

REFERENCES

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