Skip to main content Accessibility help
×
Home
Hostname: page-component-cf9d5c678-8r4lv Total loading time: 0.202 Render date: 2021-07-28T20:27:44.810Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents

Published online by Cambridge University Press:  01 June 2009

Tuula Honkenen-Buzalski
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland
Markus Sandholm
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland

Summary

The trypsin-inhibitor capacity of bovine milk was seen to increase in mastitits. This capacity showed good correlation with the California Mastitis Test score, somatic cell count (Coulter Counter method) and bovine serum albumin (BSA) content (radial immunodiffusion). The elevated antitrypsin level proved to be a safer indicator of mastitis than BSA alone. Using gel-filtration chromatography, the major trypsin-inhibitor activity of mastitic milk and plasma was eluted with BSA, indicating a similar mol. wt (70000). This fraction showed electrophoretic polymorphism. A large molecular weight fraction cross-reacted immunologically with human α2-macroglobulin. The antitrypsin activity in mastitic milk was clearly different from the principal activity in colostrum, which had a mol. wt of about 13000.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blombäck, B. & Hanson, L. Å. (1979). Plasma Proteins, pp. 343344. New York: Wiley.Google Scholar
Butler, J. E. (1971). Journal of Dairy Science 54, 13151316.CrossRefGoogle Scholar
Čechová, D. (1976). Methods in Enzymology 45, 806813.CrossRefGoogle Scholar
Ganrot, P. O., Laurell, C-B. & Eriksson, S. (1967) Scandinavian Journal of Clinical and Laboratory Investigation 19, 205208.CrossRefGoogle Scholar
Heimburger, N. (1975). In Proteases and Biological Control, pp. 367386 (Ed. Reich, E. et al. ) Cold Spring Harbor Laboratory.Google Scholar
Humbert, G. & Alais, C. (1979). Journal of Dairy Research 46, 559571.CrossRefGoogle Scholar
Klastrup, O. & Schmidt Madsen, P. (1974). Nordisk Veterinaermedicin 26, 197204.Google Scholar
Laskowski, M. Jr & Laskowski, M. (1951). Journal of Biological Chemistry 190, 563573.Google Scholar
Law, B. A. (1979). Journal of Dairy Research 46, 573588.CrossRefGoogle Scholar
Mancini, G., Carbonara, A. O. & Here.MANS, J. F. (1965). Immunochemistry 2, 235254.CrossRefGoogle Scholar
Mittman, C. (1972). Pulmonary Emphysema and Proteolysis. p. 1. New York/London: Academic Press.Google Scholar
Paape, M. J., Wergin, W. P., Guidry, A. J. & Pearson, R. E. (1979). Journal of Dairy Science 62, 135153.CrossRefGoogle Scholar
Piñeiro, A., Orteoa, F. & Uriel, J. (1975). Biochimica et Biophysica Acta 379, 201206.CrossRefGoogle Scholar
Sandholm, M. & Honkanen-Buzalski, T. (1979). Acta Veterinaria Scandinavica 20, 469476.Google Scholar
Schalm, O. W. (1977). Journal of the American Veterinary Medical Association 170, 11371140.Google Scholar
Schalm, O. W., Carroll, E. J. & Jain, N. C. (1971). Bovine Mastitis, p. 1. Philadelphia, Pa: Lea & Febiger.Google Scholar
Smith, A. M., Chesworth, J. M., Henderson, G. D. & Rodway, R. G. (1979). Journal of Dairy Research 46, 547550.CrossRefGoogle Scholar
Tolle, A., Zeidler, H. & Heeschen, W. (1966). Milchwissenschaft 21, 9398.Google Scholar
Travis, J., Baugh, R., Giles, P. J., Johnson, D., Bower, J. & Reilly, C. F. (1978). Neutral Proteases of Human Polymorphonuclear Leukocytes (Ed. Haveman, K. and Janoff., A.) pp. 118128, Baltimore & Munich: Urban & Schwarzenberg, Inc.Google Scholar
Von Fellenbero, R. & Horber, H. (1980). Schweizer Archiv für Tierheilkunde 122, 159168.Google Scholar
Weeke, B. (1973). Scandinavian Journal of Immunology 2, Suppl. 1, 1536.CrossRefGoogle Scholar
Weissmann, G., Korchak, H. E., Perez, H. D., Smolen, J. E., Goldstein, I. M. & Hoffstein, S. T. (1979). Advances in Inflammation Research 1, pp. 95112, New York: Raven Press.Google Scholar
52
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *