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Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  08 August 2018

Mengqi Wang ,
Hailiang Song ,
Xiaorui Zhu ,
Shiyu Xing ,
Meirong Zhang ,
Huimin Zhang ,
Xiaolong Wang ,
Zhangping Yang ,
Xiangdong Ding  and
Niel A. Karrow
...Show all authors
Mengqi Wang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Hailiang Song
Affiliation:
Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Xiaorui Zhu
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Shiyu Xing
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Meirong Zhang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Huimin Zhang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Xiaolong Wang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Zhangping Yang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Xiangdong Ding
Affiliation:
Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Niel A. Karrow
Affiliation:
Department of Animal Biosciences, Center for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Sven König
Affiliation:
Institute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, Ludwigstr. 21b, Gießen D-35390, German
Yongjiang Mao*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China Department of Animal Biosciences, Center for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, Canada Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, P. R. China
*
*For correspondence; e-mail: cattle@yzu.edu.cn
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Abstract

The research reported in this Research Communication aimed to describe the influence of Toll-like receptor 4 gene polymorphisms on milk production traits in Chinese Holstein cows. Toll-like receptor 4 (TLR4) is an important member of the toll-like receptor gene family that is widely found in various organisms. Since TLR4 can identify molecular patterns from various pathogenic microorganisms and induce natural and acquired immunity, it plays an important role in disease resistance in dairy cows. Two single nucleotide polymorphisms (SNPs) of TLR4 (c.−226 G > C and c.2021 C > T) that were previously found to be associated with health traits were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) for Chinese Holstein cows (n = 866). The associations between SNPs or their haplotypes and milk production traits and somatic cell count were analyzed by the generalized linear model procedure of Statistics Analysis System software (SAS). The c.−226 G > C and c.2021 C > T showed low linkage disequilibrium (r2 = 0·192). There was no association between these two SNPs and SCC, but significant effects were found for SNP c.−226 G > C on test-day milk yield, fat content, protein content, and total solid and milk urea nitrogen (P < 0·05), and SNP c.2021 C > T and the SNP haplotypes on test-day milk yield, fat content, protein content, lactose content and total solids (P < 0·05). The software MatInspector revealed that c.−226 G > C was located within several potential transcription factor binding sites, including transcription factor AP-2. The polymorphisms c.−226 G > C and c.2021 C > T had significant effects on the milk production for Chinese Holstein, and these SNP could be used for molecular marker-assisted selection of milk production.

Keywords

Toll-like receptor 4single nucleotide polymorphismsSomatic Cell Countmilk production traits
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 4 , November 2018 , pp. 407 - 411
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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Footnotes

†

These authors contributed equally to this work.

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