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Indirect assay of milk clotting activity of Mucor miehei proteinase (Fromase) in combined rennets

Published online by Cambridge University Press:  01 June 2009

Eva Beránková
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Pavel Rauch
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Jan Káš
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Vladimír Hušek
Affiliation:
Dairy Research Institute, 198 00 Prague 9, Czechoslovakia
Eduard Paluska
Affiliation:
Institute of Hematology and Blood Transfusion, 128 20 Prague 2, Czechoslovakia

Summary

Milk clotting activity of Mucor miehei proteinase (commercial name Fromase) was determined in combined rennets as the difference between the total activity of combined rennet and the residual activity after inhibition of Fromase by the purified immunoglobulin fraction of a Fromase antiserum. The proposed method is simple, rapid, and makes possible the use of any arbitrary method for assaying milk clotting activity. The application of the immunoglobulin fraction, instead of the whole antiserum, is necessary owing to the presence of nonspecific proteinase inhibitors in blood serum.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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