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Immunomodulatory activities of whey fractions in efferent prefemoral lymph of sheep

Published online by Cambridge University Press:  01 June 2009

Chun W. Wong
Affiliation:
CSIRO Division of Animal Health, Armidale, NSW 2350, Australia
Geoffrey O. Regester
Affiliation:
CSIRO Dairy Research Laboratory, Highett, VIC 3190, Australia
Geoffrey L. Francis
Affiliation:
CSIRO Division of Human Nutrition, Kintore Avenue, Adelaide, SA 5000, Australia
Dennis L. Watson
Affiliation:
CSIRO Division of Animal Health, Armidale, NSW 2350, Australia

Summary

Studies on the immunomodulatory activities of ruminant milk and colostral whey fractions were undertaken. By comparing with boiled colostral whey in a preliminary experiment, a putative heat-labile immunostimulatory factor for antibody responses was found to be present in ovine colostral whey. Studies were then undertaken in sheep in which the efferent prefemoral lymphatic ducts were cannulated bilaterally, and immune responses in the node were measured following subcutaneous injection in the flank fold of whey protein preparations of various purities. A significant sustained decline of efferent lymphocyte output was observed following injection with autologous crude milk whey or colostral whey preparations, but no changes were observed in interferon-gamma levels in lymph plasma. Two bovine milk whey fractions (lactoperoxidase and lactoferrin) of high purity were compared in bilaterally cannulated sheep. A transient decline over the first 6 h was seen in the efferent lymphocyte output and lymph flow rate after injection of both fractions. A significant difference was seen between the two fractions in interferongamma levels in lymph at 6 h after injection. However, no significant changes in the proportion of the various efferent lymphocyte phenotypes were seen following either treatment. Whereas both fractions showed a significant inhibitory effect in a dose-dependent manner on the proliferative response of T lymphocytes, but not B lymphocytes, to mitogenic stimulation in vitro, no similar changes were seen following in vivo stimulation with these two fractions.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

Abernethy, N. J. & Hay, J. B. 1992 The recirculation of lymphocytes from blood to lymph: physiological considerations and molecular mechanisms. Lymphology 25 130Google ScholarPubMed
Barta, O., Barta, V. D., Crisman, M. V. & Akers, R. M. 1991 Inhibition of lymphocyte blastogenesis by whey. American Journal of Veterinary Research 52 247253Google ScholarPubMed
Bounous, G. & Gold, P. 1991 The biological activity of undenatured dietary whey proteins: role of glutathione. Clinical and Investigative Medicine 14 296309Google ScholarPubMed
Bounous, G. & Kongshavn, P. A. L. 1985 Differential effect of dietary protein type on the B-cell and T-cell immune responses in mice. Journal of Nutrition 115 14031408CrossRefGoogle ScholarPubMed
Bounous, G., Kongshavn, P. A. L. & Gold, P. 1988 The immunoenhancing property of dietary whey protein concentrate. Clinical and Investigative Medicine 11 271278Google ScholarPubMed
Drew, P. A., Petrucco, O. M. & Shearman, D. J. C. 1983 Inhibition by colostrum of the responses of peripheral blood mononuclear cells to mitogens. Australian Journal of Experimental Biology and Medical Sciences 61 451460CrossRefGoogle ScholarPubMed
Duncan, R. L. & McArthur, W. P. 1981 Lactoferrin-mediated modulation of mononuelear cell activities. 1. Suppression of the murine in vitro primary antibody response. Cellular Immunology 63 308320CrossRefGoogle Scholar
Flat, A. M., Migliore-Samour, D., Jollès, P., Drouet, L., Sollier, C. B. D. & Caen, J. 1993 Biologically active peptides from milk proteins with emphasis on two examples concerning antithrombotic and immunomodulating activities. Journal of Dairy Science 76 301310Google Scholar
Francis, G. L., Regester, G. O., Webb, H. A. & Ballard, M. R. 1995 Extraction from cheese whey by cation-exchange chromatography of factors that stimulate the growth of mammalian cells. Journal of Dairy Science 78 12091218CrossRefGoogle ScholarPubMed
Gill, H. S., Watson, D. L. & Brandon, M. R. 1992 In vivo inhibition by a monoclonal antibody to CD4+ T cells of humoral and cellular immunity in sheep. Immunology 77 3842Google Scholar
Hall, J. G. 1967 A method for collecting lymph from the prefemoral lymph node of unanesthetized sheep. Quarterly Journal of Experimental Physiology 52 200205CrossRefGoogle Scholar
Hall, J. G. & Morris, B. 1965 The immediate effect of antigens on the cell output of a lymph node. British Journal of Experimental Pathology 46 450454Google ScholarPubMed
Juto, P. 1985 Human milk stimulates B cell function. Archives of Disease in Childhood 60 610613CrossRefGoogle ScholarPubMed
McConnell, I. & Hopkins, J. 1981 Lymphocyte traffic through antigen-stimulated nodes. I. Complement activation within lymph nodes initiates cell shutdown. Immunology 42 217223Google ScholarPubMed
McIntosh, G. H., Regester, G. O. & Smithers, G. W. 1994 Dairy foods and cancer prevention. Proceedings, International Dairy Congress 24, Melbourne, Abstracts of Posters and Brief Communications 314Google Scholar
Mincheva-Nilsson, L., Hammarström, M. L., Juto, P. & Hammarström, S. 1990 Human milk contains proteins that stimulate and suppress T lymphocyte proliferation. Clinical and Experimental Immunology 79 463469CrossRefGoogle ScholarPubMed
Miyasaka, M. & Trnka, Z. 1986 Lymphocyte migration and differentiation in a large-animal model: the sheep. Immunological Reviews. 91 87114CrossRefGoogle Scholar
Newby, T. J., Stokes, C. R. & Bourne, F. J. 1982 Immunological activities of milk. Veterinary Immunology and Immunopathology 3 6794CrossRefGoogle ScholarPubMed
Ogra, S. S. & Ogra, P. L. 1978 Immunologic aspects of human colostrum and milk. II. Characteristics of lymphocyte reactivity and distribution of E-rosette forming cells at different times after the onset of lactation. Journal of Pediatrics 92 550555CrossRefGoogle ScholarPubMed
Politis, I., Zhao, X., McBride, B. W. & Burton, J. H. 1991 Effect of bovine skim milk and whey on monocyte function. Journal of Dairy Science 74 24672471CrossRefGoogle ScholarPubMed
Rothel, J. S., Jones, S. L., Corner, L. A., Cox, J. C. & Wood, P. R. 1990 A sandwich enzyme immunoassay for bovine interferon-gamma and its use for the detection of tuberculosis in cattle. Australian Veterinary Journal 67 134137CrossRefGoogle ScholarPubMed
Sarfati, M., Vanderbeeken, Y., Rubio-Trujillo, M., Duncan, D. & Delespesse, G. 1986 Presence of IgE suppressor factors in human colostrum. European Journal of Immunology 16 10051008CrossRefGoogle ScholarPubMed
Shimizu, M. 1994 Bioactive peptide from bovine milk proteins. Proceedings, International Dairy Congress 24, Melbourne, Abstracts of Posters and Brief Communications 26Google Scholar
Stoeck, M., Ruegg, C., Miescher, S., Carrel, S., Cox, D., von Fliedner, V. & Alkan, S. 1989 Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors β1 and β2. Journal of Immunology 143 32583265Google Scholar
Watson, D. L. 1990 Immunological activity of factors in colostrum and milk. In Joint Convention Papers, Gold Coast, QLD, 6th–10th May, pp. 8183 (Eds Halais, C., Deeth, H., Fedrick, I., Jehne, C., Leith, G., Macfarlane, J. and Paroz, P.). The QLD Branch of The Australian and New Zealand Institutes of Food Science and Technology LtdGoogle Scholar
Watson, D. L., Francis, G. L. & Ballard, F. J. 1992 Factors in ruminant colostrum that influence cell growth and murine IgE antibody responses. Journal of Dairy Research 59 369380CrossRefGoogle ScholarPubMed
Wong, C. W. & Watson, D. L. 1995 Immunomodulatory effects of dietary whey proteins in mice. Journal of Dairy Research 62 359368CrossRefGoogle ScholarPubMed

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