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High-level coproduction of the bacteriocins nisin A and lactococcin A by Lactococcus lactis

Published online by Cambridge University Press:  04 May 2004

Antonio Fernández
Affiliation:
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
Nikki Horn
Affiliation:
Food Safety Science Division, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Michael J Gasson
Affiliation:
Food Safety Science Division, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Helen M Dodd
Affiliation:
Food Safety Science Division, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Juan M Rodríguez
Affiliation:
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain

Abstract

In this study, a two-plasmid system for enhanced and consistent biosynthesis of the model lactococcal bacteriocin lactococcin A in non-producing Lactococcus lactis hosts was developed. The system comprised a plasmid carrying the genes lcnA and lciA under the control of the nisin-inducible nisA promoter, and a second plasmid harbouring the lcnC and lcnD genes. The introduction of both plasmids into two strains containing the nisRK genes required for nisin-controlled expression, Lc. lactis FI5876 (a nisin A-producer strain) and FI7847, resulted in production of extracellular lactococcin A at a higher level than that for the parental strain, Lc. lactis WM4. In addition, transformation of the nisin-producing host with both plasmids led to a high-level production of both lactococcal bacteriocins, which may provide a means to exploit their complementary properties in cheese ripening.

Type
Research Article
Copyright
Proprietors of Journal of Dairy Research 2004

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